Preparation and characterisation of composite nanomaterials for radionuclide delivery

master's thesis

• Title (croatian): Priprava i karakterizacija kompozitnih nanomaterijala za dostavu radionuklida
• Author: Natalija Pantalon Juraj
• Mentor: Ivana Steinberg (mentor)
• Committee member: Ivana Steinberg (predsjednik povjerenstva)
• Committee member: Stanislav Kurajica (član povjerenstva)
• Committee member: Svjetlana Krištafor (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2016-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Applied Chemistry

Abstract

The aim of this thesis was to prepare composite nanomaterials for targeted delivery of radionuclides used for cancer diagnosis and therapy. Hydroxyapatite and TiO2 nanoparticles were used as vehicles for targeted delivery because of their suitable properties such as biocompatibility and radiochemical stability. Nanocomposites were prepared by precipitating phosphonate ligands or polyethylene glycol with nanoparticles. The role of the phosphonates is binding of radionuclides and stabilisation of nanoparticles. Prepared nanocomposites were labelled with 99mTc, a gamma emmiter used in tumor imaging and 223Ra, an alpha emmiter used in targeted alpha therapy. Phosphonic acids ethylenediamine tetra(methylenephosphonic) acid, propylenediamine tetra(methylenephosphonic) acid and 1,2-cyclohexyldiamine tetra(methylenephosphonic) acid were purified by ion exchange chromatography and used for preparation of nanocomposites. Besides mentioned ligands, methylene diphosphonic acid, etidronic acid, zoledronic acid, pamidronic acid and polyethylene glycol were used for experiments. For labelling with 99mTc, the best yields were obtained for 99mTc-CDTMP-HAp (89%), and 99mTc-PDTMP-HAp (93%) stabilised before labelling and 99mTc-MDP-HAp (92%) and 99mTc-HEDP-HAp (89%) stabilised during their preparation. TiO2 nanocomposites all had yields >93%. Nanocomposites 223Ra-zoledronic acid-HAp stabilised after labelling showed the best yields (60%) for labelling with 223Ra.

Abstract (croatian)

Cilj ovog rada bio je priprema kompozitnih nanomaterijala za ciljanu dostavu radionuklida koji se koriste u dijagnostici i terapiji raka. Nanočestice hidroksiapatita i TiO2 korištene su kao nosači za ciljanu dostavu zbog njihovih pogodnih svojstava, kao što su biokompatibilnost i radiokemijska stabilnost. Nanokompoziti su pripravljeni miješanjem fosfonatnih liganda ili polietilen glikola s nanočesticama. Uloga fosfonata je vezanje radionuklida i stabilizacija nanočestica. Pripremljeni nanokompoziti obilježeni su s 99mTc, radionuklidom koji emitira gama zračenje i koristi se u dijagnostici tumora te 223Ra, koji emitira alfa zračenje i koristi se u ciljanoj alfa terapiji. Fosfonske kiseline, etilendiamin tetra(metilenfosfonska) kiselina, propilendiamin tetra(metilenfosfonska) kiselina i 1,2-cikloheksildiamin tetra(metilenfosfonska) kiselina, pročišćene su ionsko izmjenjivačkom kromatografijom i korištene su za pripravu nanokompozita. Osim navedenih liganda, u eksperimentima su korištene i metilen difosfonska, etidronska, zoledronska i pamidronska kiselina te polietilen glikol. Za obilježavanje s 99mTc, najbolje iskorištenje dobiveno je za 99mTc-CDTMP-HAp (89%), i 99mTc-PDTMP-HAp (93%) stabilizirane prije označavanja i 99mTc-MDP-HAp (92%) i 99mTc-HEDP-HAp (89%) stabilizirane tijekom sinteze nanočestica. Svi nanokompoziti TiO2 imali su iskorištenje veće od 93%. Nanokompoziti 223Ra-zoledronska kiselina-HAp stabilizirani nakon obilježavanja pokazali najbolje iskorištenje (60%) za označavanje s 223Ra.

• Keywords: nanocomposites; hydroxyapatite; TiO2; phosphonic acids; 99mTc; 223Ra
• Keywords (croatian): nanokompoziti; hidroksiapatit; TiO2; fosfonske kiseline; 99mTc; 223Ra
• Language: english
• URN:NBN: urn:nbn:hr:149:023711
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Advanced materials and technologies; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-05 13:19:36