Ispitivanje utjecaja aktivacijskih agensa na uspješnost procesa imobilizacije enzima na mezoporoznu siliku

master's thesis

• Title (english): Examination of the impact of activation agents on enzyme immobilisation on mesoporous silica
• Author: Ivana Bukarica
• Mentor: Ana Vrsalović Presečki (mentor)
• Committee member: Ana Vrsalović Presečki (predsjednik povjerenstva)
• Committee member: Vanja Kosar (član povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2020-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

U ovom radu provedena je kovalentna imobilizacija biokatalizatora DERA na MCF mezoporoznu siliku funkcionaliziranu APTES-om, te aktiviranu benzokinonom u svrhu povećanja njegove stabilnosti. Mezoporozna silika sintetizirana je sol – gel metodom. Aktivnost biokatalizatora ispitana je u reakciji dvostruke aldolne adicije acetaldehida i kloroacetaldehida koja kao produkt daje laktol, važan prekursor u sintezi statina. Ispitao se utjecaj procesa aktivacije nosioca na uspješnost imobilizacije biokatalizatora korištenjem različitih koncentracija benzokinona. Rezultati su pokazali da se pri koncentraciji benzokinona od 3 mM postižu optimalne vrijednosti parametara uspješnosti imobilizacije. Također je ispitan utjecaj temperature i vremena imobilizacije na način da su ispitivanja provedena pri temperaturama od 15 - 40 °C te pri vremenima imobilizacije od 2 - 4 h. Temperatura od 25 °C se pokazala kao optimalna, a također je zaključeno kako vrijeme trajanja imobilizacije nema značajnog utjecaja na uspješnost samog procesa.

Abstract (english)

In this work, the covalent immobilisation of the biocatalyst DERA in order to increase its stability was done. As a carrier MCF mesoporous silica functionalised by APTES and activated by benzoquinone was used. Mesoporous silica was synthesised by the sol–gel method. Biocatalyst activity was examined in the reaction of double aldol addition of acetaldehyde and chloroacetaldehyde, which results in lactol, an important precursor in the statin synthesis. The influence of different concentrations of benzoquinone in the activation process on the success of biocatalyst immobilization was examined. Results have shown that benzoquinone concentration of 3 mM was optimal. Also, the impact of temperature in the range of 15 - 40 °C and the immobilisation time (2 - 4 h) were investigated. Temperature of 25 °C proved to be optimal, and it was also concluded that the duration of immobilisation process had no significant impact on the success of the process.

• Keywords: DERA; biokatalizator; imobilizacija; mezoporozna silika; aldolna adicija
• Keywords (english): DERA; biocatalyst; immobilisation; mesoporous silica; aldol addition
• Language: croatian
• URN:NBN: urn:nbn:hr:149:389738
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2022-02-28 17:59:22