Priprava i karakterizacija polimernih mikročestica

undergraduate thesis

• Title (english): Preparation and characterisation of polymeric microparticles
• Author: Zoran Malbaša
• Mentor: Anamarija Rogina (mentor)
• Committee member: Anamarija Rogina (predsjednik povjerenstva)
• Committee member: Marica Ivanković (član povjerenstva)
• Committee member: Leonard Bauer (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-09-21, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Postoji nekoliko tehnika korisnih za pripremu biorazgradljivih polimernih čestica na temelju kitozana. Priprema mikročestica jednake veličine s kontroliranom morfologijom još uvijek je izazov. Neke od najčešće upotrebljavanih metoda za dobivanje mikročestica su metode temeljene na pripravi emulzija, proces elektroštrcanja te metoda sušenja raspršivanjem. Metoda koja se koristila u ovom radu, upravo zbog svoje jednostavnosti i velike efikasnosti je elektroštrcanje. Ovaj rad istražuje utjecaj dodatka iona bakra (Cu2+) i cinka (Zn2+) na veličinu, oblik i mikrostrukturu mikročestica kompleksa metalnih iona i kitozana (Cu2+/Zn2+‒CHT85) dobivenih elektroštrcanjem. Morfologija mikrosfera Cu2+/Zn2+‒CHT85 ispitana je pretražnim elektronskim mikroskopom, čime je potvrđena sferičnost mikročestica uočena primjenom optičkog mikroskopa. Veličina i raspodjela čestica ovisi o koncentraciji dodanih metalnih iona te je procijenjeni promjer mikrosfera Cu/Zn0,5‒CHT85 i Cu/Zn5‒CHT85 iznosio 80 ± 22 μm, odnosno 107 ± 32 μm. Za identifikaciju suhih mikrosfera korištena je infracrvena spektroskopija s Fourierovom transformacijom prigušene totalne refleksije (ATR-FTIR) gdje nije uočena pojava novih vrpci, već samo promjene u intenzitetima i valnim brojevima apsorpcijskih vrpci amino i hidroksilnih skupina, amida I i amida II koje mogu ukazivati na to da atomi dušika i kisika u polimeru koordiniraju bakrove i cinkove ione. Primjenom metode elektroštrcanja mogu se uspješno dobiti mikrosfere na temelju kitozana.

Abstract (english)

There are several techniques useful for preparing biodegradable chitosan-based polymer particles. The preparation of microparticles of uniform size with controlled morphology is still a challenge. Some of the most commonly used methods for obtaining microparticles are methods based on the preparation of emulsions, the electrospraying process and the spray drying method. Due to simplicity and high efficiency, electrospraying process was used in this work. This work investigates the influence of the addition of copper (Cu2+) and zinc (Zn2+) ions on the size, shape and microstructure of microparticles of the bimetallic ion–chitosan complex (Cu2+/Zn2+‒CHT85) obtained by electrospraying. The morphology of the Cu2+/Zn2+‒CHT85 microspheres was investigated by a scanning electron microscope, which confirmed the sphericity of the microparticles observed using an optical microscope. The size and distribution of the particles depends on the concentration of metal ions, and estimated diameter of microspheres Cu/Zn0.5‒CHT85 and Cu/Zn5‒CHT85 was 80 ± 22 μm and 107 ± 32 μm, respectively. The identification of dry microspheres was carried out by Fourier infrared spectroscopy (ATR-FTIR) which indicated the absence of new absorption bands and slight changes in the intensities and wave numbers of the absorption bands of amino and hydroxyl groups, amide I and amide II. These changes could indicate that copper and zinc ions are coordinated by the nitrogen and oxygen atoms in the polymer. Chitosan-based microspheres can be successfully obtained by electrospraying method.

• Keywords: kitozan; mikrosfere; elektroštrcanje; Cu2+ i Zn2+ ioni; kompleks
• Keywords (english): chitosan; microspheres; electrospray; Cu2+ and Zn2+ ions; complex
• Language: croatian
• URN:NBN: urn:nbn:hr:149:948806
• Project: Number: UIP-2020-02-6201; Title: Pametni sustavi za dostavu lijeka pri liječenju tumora kosti; Title: Smart drug delivery systems in bone tumour treatment; Acronym: SmartCar; Leader: Anamarija Rogina; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: UIP
• Study programme: Title: Materials Science and Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2023-03-06 13:21:59