Ispitivanje otpuštanja konstituenata mikroplastike u vodenom mediju

undergraduate thesis

• Title (english): Leaching of microplastic constituents in aqueous medium
• Author: Filip Mikuličić
• Mentor: Ana Lončarić Božić (mentor)
• Committee member: Ana Lončarić Božić (predsjednik povjerenstva)
• Committee member: Ana Vrsalović Presečki (član povjerenstva)
• Committee member: Marin Kovačić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Već nekoliko desetljeća mikroplastika se zamjećuje u okolišu u koji dospijeva putem otpadnih voda ili u njemu nastaje degradacijom plastičnog otpada pod utjecajem fizikalnih ili kemijskih sila. Prilikom proizvodnje plastike koriste se razni aditivi i katalizatori koji mogu biti otpušteni u okoliš tijekom procesa degradacije čestica plastike i kao takvi predstavljaju moguće onečišćenje okoliša. Zbog značajne količine proizvedene plastike u svijetu potrebno je istražiti ponašanje otpuštanja takvih tvari u vodeni okoliš i njihov utjecaj na žive organizme. Cilj ovog rada je korištenje analitičkih i bioanalitičkih metoda za određivanje brzine otpuštanja i koncentracije aditiva bisfenola A i katalizatora antimona iz UV-starenih i nestarenih uzoraka PET plastike u vodenom mediju. Također, praćen je i utjecaj toksičnosti otpuštenih tvari na bioluminiscentne bakterije Vibrio fischeri mjerenjem vrijednosti EC50, EC20 i EC10. Provedena ispitivanja pokazala su pojavu otpuštanja bisfenola A u vodeni medij iz starenih i nestarenih uzoraka, pri čemu je otpuštanje bilo brže iz UV-starenih uzoraka. Zamjećeno je i otpuštanje antimona, no u mnogo manjoj količini kod UV-starenih uzoraka. Uočen je i inhibicijski utjecaj otopine dobivene izluživanjem bisfenola A i antimona iz PET uzoraka na bakterije Vibrio fischeri. Koncentracija bisfenola A praćena je tekućinskom kromatografijom visoke djelotvornosti, a antimona induktivno spregnutom plazmom s masenim spektrometrom. Za mjerenje ekotoksičnosti korištena je standardna metoda ISO 11348-2:2007.

Abstract (english)

Since several decades by now, microplastics has been detected in the environment which it reaches either by wastewater or is being created within from degradation of plastic waste by influence of physical or chemical environmental forces. During the plastics production many various additives and catalysts are being used which may leak into the environment during the plastics' degradation and as such they present possible polluting effect. Due to significant amounts of plastics produced in the world, it is necessary to research the behaviour of the release of such chemicals into the aquatic environment and their possible impact on living organisms. The aim of this work is to use analytical and bioanalytical methods to determine the rate of release and concentration of the additive bisphenol A and antimony catalyst from UV-aged and non-aged PET plastic samples in an aqueous medium. Likewise, the influence of the toxicity of the released substances on the bioluminescent bacteria Vibrio fischeri was monitored by measuring the values of EC50, EC20 and EC10. The conducted tests showed the release of bisphenol A and antimony into the aqueous medium from both UV-aged and unaged PET samples. The release of antimony was also observed, albeit its amount was much lower from the UV-aged sample. Leached samples with Antimony and bisphenol A also showed an inhibitory effect on Vibrio fischeri bacteria. The concentrations of bisphenol A and antimony were monitored by high performance liquid chromatography and inductively coupled plasma with a mass spectrometer respectively. The standard method ISO 11348-2:2007 was used to measure ecotoxicity.

• Keywords: Bisfenol A; mikroplastika; antimon; tekućinska kromatografija; Vibrio fischeri
• Keywords (english): Bisphenol A; microplastics; antimony; liquid chromatography; Vibrio fischeri
• Language: croatian
• URN:NBN: urn:nbn:hr:149:330345
• Project: Number: IP-2020-02-6033; Title: Mikroplastika u vodi; sudbina, ponašanje i uklanjanje; Title: Microplastics in water; fate and behavior and removal; Acronym: ReMiCRO; Leader: Ana Lončarić Božić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Environmental Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2023-03-08 13:18:21