Praćenje razgradnje odabrane aktivne supstance

master's thesis

• Title (english): Monitoring of degradation of selected active substance
• Author: Jelena Kovačić
• Mentor: Šime Ukić (mentor)
• Committee member: Šime Ukić (predsjednik povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Committee member: Matija Cvetnić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-07-01, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Prisutnost novih onečišćivala u okolišu, konkretno farmaceutika i njihovih razgradnih (među)produkata, znatno zabrinjava javnost zbog njihova potencijalno štetnog djelovanja. Njihova pojava zabilježena je u raznim sustavima, poput postrojenja za obradu otpadnih voda, površinskim vodama, morskoj vodi, podzemnoj vodi, te tlu i sedimentu. Većina konvencionalnih metoda njihovog uklanjanja nije dovoljno djelotvorna budući da su farmaceutici uglavnom topivi u vodi i slabo biorazgradivi. Cilj ovog rada bio je pratiti tijek razgradnje odabrane aktivne supstance tekućinskom kromatografijom visoke djelotvornosti spregnutom sa spektrometrom masa kao detektorom. Aktivna supstanca podvrgnuta je razgradnji kroz sljedeće procese: hidrolizu, fotolizu, prisilnu razgradnju oksidansom, kiselinom i lužinom te UV-C fotooksidaciju uz primjenu H2O2 i Na2S2O8 kao oksidansa. Najučinkovitijim tehnikama uklanjanja pokazali su se napredni oksidacijski procesi među kojima se ističu fotooksidacijski procesi s H2O2 te prisilna razgradnja oksidansom i kiselinom. Odabrana kromatografska metoda je validirana. Razlučivanje svih pikova bilo je veće od 1,5 što govori u prilog visokoj selektivnost metode, linearnost odziva potvrđena je R^2 vrijednošću većom od 0,99, analitički povrat od 102,35% govori u prilog točnosti metode, dok u prilog visoke preciznosti metode govori RSD vrijednost manja od 2%.

Abstract (english)

The presence of new pollutants in the environment, namely pharmaceuticals and their degradation (by)products, has become a matter of considerable concern due to their potentially harmful effects. Their occurrence has been reported in wastewater treatment plants, surface waters, seawater, groundwater, soil and sediment. Most conventional methods for their removal are insufficiently effective as pharmaceuticals are mostly water soluble and poorly biodegradable. The aim of this study was to monitor the degradation of the selected active substance by high performance liquid chromatography coupled with mass spectrometry. The active substance was subjected to degradation through the following processes: hydrolysis, photolysis, forced degradation with oxidant, acid and base and UV-C photooxidation with H2O2 and Na2S2O8 as oxidants. The results pointed out UV-C/H2O2 process, forced oxidant degradation and degradation in acidic conditions as the most effective removal approaches. The selected chromatographic method was validated. Resolution values above 1.5 proved high selectivity of the method, while R^2 values confirmed good linearity with values above 0.99. Acceptable accuracy and precision of the method were indicated by recovery of 102.25% and RSD value below 2%.

• Keywords: farmaceutici; okoliš; razgradnja; kromatografija
• Keywords (english): pharmaceuticals; environment; degradation; chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:149:397846
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Applied organic chemistry; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2023-04-05 13:53:39