Biotransformacija stirena u stiren oksid in vitro

master's thesis

• Title (english): Biotransformation of styrene to styrene oxide in vitro
• Author: Milan Vukšić
• Mentor: Đurđa Vasić-Rački (mentor)
• Committee member: Đurđa Vasić-Rački (predsjednik povjerenstva)
• Committee member: Bruno Zelić (član povjerenstva)
• Committee member: Domagoj Vrsaljko (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2015-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Reaction Engineering

Abstract

U ovom radu provedena je in vitro biotransformacija stirena u stiren oksid pročišćenim stanicama genetski modificirane bakterije Pseudomonas putida. Pokusima na tresilici ispitan je utjecaj početne koncentracije stirena i koncentracije biomase na volumnu produktivnost enzima stiren monooksigenaze i konverziju. Parametri kinetičkog modela procesa biotransformacije stirena u stiren oksid procijenjeni su na temelju rezultata pokusa provedenih u kiveti. Pri procjeni parametara kinetičkog modela korištene su simpleks metoda ili metoda najmanjih kvadrata ugrađene u programski paket SCIENTIST.
Postavljen je matematički model procesa biotransformacije stirena u stiren oksid. Rezultati simulacije matematičkog modela procesa su uspoređeni s eksperimentalnim rezultatima te je dobiveno njihovo dobro slaganje.

Abstract (english)

In this work the biotransfomation of styrene to S-styrene oxide in vitro were conducted with purified cells genetically modified bacteria Pseudomonas putida. The effect of initial concentration of styrene and concentration of biomass on volumetric productivity of enzyme styrene monooxygenase and conversion are investigated on a shaker. The kinetic parameters of the process model of biotransformation of styrene to styrene oxide were estimated from the experimental data obtained in cuvette. Kinetic model parameters were estimated by simplex or least squares method using SCIENTIST software package.
A mathematical model of biotransformation of styrene to S-styrene oxide was developed. The model fitted experimental results very well.

• Keywords: stiren; stiren oksid; stiren monooksigenaza; biotransformacija; matematički model procesa
• Keywords (english): styrene; styrene oxide; styrene monooxygenase; biotransformation; mathematical model of the process
• Language: croatian
• URN:NBN: urn:nbn:hr:149:306319
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2016-12-16 11:51:05