Priprema uzoraka aktivnog mulja za kromatografsku analizu ksenobiotika

master's thesis

• Title (english): Sample preparation of activate sludge for chromatographic analysis of xenobiotics
• Author: Ivana Hlevnjak
• Mentor: Danijela Ašperger (mentor)
• Committee member: Danijela Ašperger (predsjednik povjerenstva)
• Committee member: Martina Biošić (član povjerenstva)
• Committee member: Dragana Vuk (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Farmaceutski ksenobiotici vrsta su onečišćujućih tvari široko rasprostranjenih u sustavima za obradu otpadnih voda. Većina njih nalazi se u aktivnom mulju dobivenom u sekundardnom procesu obrade otpadnih voda. Aktivni mulj veoma je složena matica koja zahtjeva pripremu kako bi se ekstrahirali analiti i uklonile interferencije. U ovome radu za pripremu uzoraka aktivnog mulja koristila se metoda ekstrakcije raspršenjem matice uzorka kroz čvrstu fazu (MSPD). Određivanje ksenobiotika provedeno je tekućinskom kromatografijom visoke djelotvornosti s detektorom s nizom dioda (HPLC-DAD). Analizirano je sedam ksenobiotika, uključujući antibiotike (cefdinir i nitrofurantoin), antihelmintike (albendazol, febantel i prazikvantel), antiemetik (metoklopramid) i antimalarik (hidroksiklorokin). Neki od parametara MSPD ekstrakcije koji su optimizirani su vrsta sorbensa, omjer mase aktivnog mulja i sorbensa, koncentracija standardne otopine i ekstrakcijsko otapalo. Najbolji rezultati su postignuti korištenjem ljuski jajeta s omjerom mase aktivnog mulja i ljuski jajeta (1:7) i ekstrahiranjem s 5 mL smjese otapala metanol:voda (50:50).

Abstract (english)

Pharmaceutical xenobiotics are a type of pollutant widely used in wastewater treatment systems. Most of them are found in activated sludge obtained in the secondary process of wastewater treatment. Activated sludge is a very complex matrix that requires preparation to extract analytes and remove interference. In this study, the method of extraction by matrix solid-phase dispersion (MSPD) was used for the preparation of activated sludge samples. Determination of xenobiotics was performed by high performance liquid chromatography with a diode array detector (HPLC-DAD). Seven xenobiotics were analyzed, including antibiotics (cefdinir and nitrofurantoin), anthelmintics (albendazole, febantel, and praziquantel), antiemetics (metoclopramide), and antimalarials (hydroxychloroquine). Some of the MSPD extraction parameters that have been optimized are sorbent type, activated sludge to sorbent mass ratio, standard solution concentration, and extraction solvent. The best results were obtained using egg shells with a ratio of activated sludge to egg shells (1:7) and extraction with 5 mL of a solvent mixture of methanol:water (50:50).

• Keywords: ksenobiotici; aktivni mulj; raspršenje matice uzorka kroz čvrstu fazu (MSPD); tekućinska kromatografija visoke djelotvornosti s detektorom s nizom dioda (HPLC-DAD)
• Keywords (english): xenobiotics; activated sludge; matrix solid-phase dispersion (MSPD); high-performance liquid chromatography with a diode array detector (HPLC-DAD)
• Language: croatian
• URN:NBN: urn:nbn:hr:149:081536
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2023-04-17 12:15:26