Analiza učinkovitosti izoliranih mikroorganizama u bioremedijaciji nafte i naftnih produkata

master's thesis

• Title (english): Analysis of the effectiveness of the isolated microorganisms in bioremediation of oil and oil products
• Author: Ivan Zgrebec
• Mentor: Marija Vuković Domanovac (mentor)
• Committee member: Marija Vuković Domanovac (predsjednik povjerenstva)
• Committee member: Zvjezdana Findrik Blažević (član povjerenstva)
• Committee member: Monika Šabić Runjavec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Nafta predstavlja jedan od glavnih energenata današnjice i njena upotreba vrlo je široka. Svakodnevnim korištenjem i eksploatacijom nafte dolazi do neželjenog ispuštanja nafte u okoliš, što predstavlja veliki ekološki problem. Sve se više istražuju nove, „zelene“ tehnologije koje se mogu koristiti za uklanjanje naftnog onečišćenja bez negativnog utjecaja na okoliš. Bioremedijacija može uz prirodne mikroorganizme razgraditi prisutno onečišćenje. Mikroorganizmi proizvode enzime koji razgrađuju naftu i naftne derivate ili koriste naftu kao izvor energije za svoj rast i razmnožavanje. Identifikacija autohtonih mikroorganizama te poznavanje njihovih svojstava od velike je važnosti kako bi se povećala učinkovitost bioremedijacije. U ovom radu provedena je identifikacija i karakterizacija autohtonih mikrobnih kultura iz uzorka tla onečišćenog naftnim derivatima u šaržnim uvjetima i u periodu 28 dana. Dobivene vrijednosti CFU su se kretale u rangu od 6,26 × 10^4 do
7,60 × 10^8 /g tla. Rezultati pokazuju da je aktivnost bakterija i gljiva bila najveća pri pH-vrijednosti 7,8 i 7,0. Na temelju morfologije, biokemijskih testova i MALDI TOF analize identificirane su slijedeće bakterijske vrste: Lysobacter daejeonensis, Cupriavidus necator, Novosphingobium taihuense i Staphylococcus warneri.

Abstract (english)

Oil is one of the most important sources of energy today and its use is widespread. The daily use and exploitation of oil results in the unwanted release of oil into the environment, which is a major environmental problem. New "green" technologies are being explored that can eliminate oil pollution without harming the environment. Bioremediation can break down existing pollution with natural microorganisms. Microorganisms produce enzymes that can break down oil and oil derivatives or use oil as an energy source for their growth and reproduction. Identification of indigenous microorganisms and information about their properties are of great importance to increase the efficiency of bioremediation. In this study, the identification and analysis of the efficiency of microbial cultures from a soil sample contaminated with oil derivatives were carried out under batch conditions over a period of 28 days. The CFU values obtained ranged from 6.26 × 10^4 to 7.60 × 10^8 /g soil. The results show that bacterial and fungal activity was highest at pH 7.8 and 7.0, respectively. Based on morphology, biochemical tests and MALDI TOF analysis, the following bacterial species were identified: Lysobacter daejeonensis, Cupriavidus necator, Novosphingobium taihuense and Staphylococcus warneri.

• Keywords: nafta; onečišćenje tla; autohtoni mikroorganizmi; bioremedijacija
• Keywords (english): oil; soil pollution; autochthonous microorganisms; bioremediation
• Language: croatian
• URN:NBN: urn:nbn:hr:149:906848
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-10-01
• Created on: 2023-11-15 12:44:29