Uklanjanje ksenobiotika biorazgradnjom pomoću mješovite bakterijske kulture

master's thesis

• Title (english): Removal of xenobiotics by biodegradation using a mixed bacterial culture
• Author: Ana Čurić
• Mentor: Marija Vuković Domanovac (mentor)
• Committee member: Marija Vuković Domanovac (predsjednik povjerenstva)
• Committee member: Zvjezdana Findrik Blažević (član povjerenstva)
• Committee member: Monika Šabić Runjavec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-02-20, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Napredak svih ljudskih djelatnosti omogućio je bolju kvalitetu života, ali je i doveo do povećanog ispuštanja ksenobiotika u okoliš koji nepravilnim zbrinjavanjem narušavaju kakvoću okoliša. Sve češći takvi antropogeni spojevi su upravo farmaceutici. Zbog njihove sveprisutnosti u okolišu dolazi do razvijanja otpornosti na antibiotike kod različitih mikrooganizama. Kako bi se riješila problematika ksenobiotika u okolišu, primjenjuje se postupak biorazgradnje. To je ekonomičan i okolišno prihvatljiv postupak koji iskorištava prirodno urođene metaboličke i enzimatske sposobnosti mikroorganizama za razgradnju organskih onečišćujućih tvari. U ovome radu provedeno je istraživanje procesa biorazgradnje β-laktamskog antibiotika amoksicilina pomoću bakterijskih kultura Pseudomonas aeruginosa i Bacillus subtilis. Početne koncentracije amoksicilina izražene preko kemijske potrošnje kisika (KPK) iznosile su 278 mg L^-1 za pokus P1 i 658 mg L^-1 za pokus P2. Za uvid u učinkovitost biorazgradnje provedeni su testovi osjetljivosti i mikroskopska analiza. Učinkovitost procesa biorazgradnje iznosila je za P. aeruginosa 49,6 % i 36,2 % i za B. subtilis 99,7 % i 48,5 % u pokusima P1 i P2.

Abstract (english)

The progress in all human activities has ensured a better quality of life, but it has also led to increased release of xenobiotic compounds into the environment. Due to their improper disposal, they disrupt environmental quality. Such anthropogenic compounds, increasingly present in the environment, are pharmaceuticals. Their ubiquitous presence in the environment contributes to the development of antibiotic resistance in various microorganisms. To solve the problem of xenobiotics in the environment, the biodegradation process is used. This is an economical and environmentally friendly process that utilizes the natural metabolic and enzymatic abilities of microorganisms to degrade organic pollutants. In this study, research was conducted on the biodegradation process of the β-lactam antibiotic amoxicillin using bacterial cultures of Pseudomonas aeruginosa and Bacillus subtilis. Initial concentrations of amoxicillin, expressed in terms of chemical oxygen demand (COD) values, were 278 mg L^-1 and 658 mg L^-1 for experiments P1 and P2. To gain insight into the efficiency of biodegradation, sensitivity tests and microscopic analyses were performed.
The biodegradability was 49.6 % and 36.2 % for P. aeruginosa and 99.7 % and 48.5 % for
B. subtilis in trials P1 and P2, respectively.

• Keywords: ksenobiotik; biorazgradnja; amoksicilin; Pseudomonas aeruginosa; Bacillus subtilis
• Keywords (english): xenobiotic; biodegradation; amoxicillin; Pseudomonas aeruginosa; Bacillus subtilis
• Language: croatian
• URN:NBN: urn:nbn:hr:149:719742
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-10-01
• Created on: 2024-02-21 11:03:55