Poboljšanje bioremedijacije otpadnog toka egzogenim mikrobnim kulturama

master's thesis

• Title (english): Improving bioremediation of waste streams through exogenous microbial cultures
• Author: Mirela Volf
• Mentor: Marija Vuković Domanovac (mentor)
• Committee member: Marija Vuković Domanovac (predsjednik povjerenstva)
• Committee member: Monika Šabić Runjavec (član povjerenstva)
• Committee member: Ante Vučemilović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-04-30, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

U modernom svijetu tehnološkog napretka, antropogene aktivnosti dovode do sve većeg onečišćenja voda. Procjedne vode koje nastaju iz organske frakcije miješanog komunalnog otpada sadrže visoko opterećenje te zahtijevaju fizikalnu, kemijsku ili biološku obradu prije ispuštanja u okoliš. Tehnologija bioremedijacije postaje sve važnija u suočavanju s navedenim izazovima jer pruža održivo i visokoučinkovito rješenje za sanaciju različitih vrsta onečišćenja. Onečišćujuće tvari putem procesa biorazgradnje ili biotransformacije prevode se u netoksične produkte i nusprodukte djelovanjem enzimskog kataboličkog potencijala mikroorganizama. Kako bi se povećala brzina i djelotvornost tehnologije bioremedijacije uvode se dodatni alati kao što su optimiranje procesnih čimbenika putem biostimulacije te korištenje autohtonih ili egzogenih kultura kao inokuluma u procesu bioaugmentacije.
U ovom radu istraživana je bioremedijacija procjedne vode iz organske frakcije iz miješanog komunalnog otpada. Uspoređena je djelotvornost uklanjanja organskog onečišćenja uz bioaugmentaciju egzogenih mikrobnih kultura Pseudomonas putida te aktivnog mulja.
Pokusi su provedeni u šaržnom reaktoru pri submerznim uvjetima u periodu od 10 dana. Ukupna prosječna vrijednost učinkovitosti procesa uklanjanja organske tvari za istraživane sustave iznosila je 89 %. Provedena istraživanja pružaju uvide u praktičnu primjenu bioremedijacije i doprinose daljnjem razvoju strategija za obradu otpadnih voda.

Abstract (english)

In the modern society of technological progress, anthropogenic activities are leading to increasing water pollution. Leachates originating from the organic fraction of mixed municipal waste are highly contaminated and require physical, chemical or biological treatment before being discharged into the environment. Bioremediation technology is becoming increasingly important in overcoming these challenges as it offers a sustainable and highly effective solution for the remediation of various types of pollution. Pollutants, through processes of biodegradation or biotransformation, are converted into non-toxic products and byproducts through the enzymatic catabolic potential of microorganisms. To enhance the speed and effectiveness of bioremediation technology, additional tools are introduced, such as the optimisation of process factors through biostimulation and the use of indigenous or exogenous cultures as inoculants in bioaugmentation. In this work, the bioremediation of leachate from the organic fraction of mixed municipal waste was investigated.
The effectiveness of the removal of organic pollution removal with bioaugmentation with exogenous microbial cultures Pseudomonas putida and activated sludge was compared.
The experiments were carried out in a batch reactor under submerged conditions for 10 days. The average overall efficiency of the organic matter removal in the investigated systems was 89 %. The conducted research provides insights into the practical application of bioremediation and contributes to the further development of wastewater treatment strategies.

• Keywords: procjedne vode; tehnologija bioremedijacije; bioaugmentacija; biostimulacija; NATO doktrine o zaštiti okoliša
• Keywords (english): leachate waters; bioremediation technology; bioaugmentation; biostimulation; NATO environmental protection doctrines
• Language: croatian
• URN:NBN: urn:nbn:hr:149:063274
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-03-30
• Created on: 2024-05-06 11:56:20