Kvalitativna analiza fenolnih spojeva u komini jabuke tekućinskom kromatografijom

undergraduate thesis

• Title (english): Qualitative analysis of phenolic compounds in apple pomace by liquid chromatography
• Author: Mihaela Voća
• Mentor: Dajana Kučić Grgić (mentor)
• Committee member: Dajana Kučić Grgić (predsjednik povjerenstva)
• Committee member: Martina Miloloža (član povjerenstva)
• Committee member: Josipa Papac Zjačić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Jabuka je jedna od najrasprostranjenijih voćnih vrsta u svijetu i koristi se za svježu konzumaciju i proizvodnju različitih prerađevina. Prilikom prerade jabuka nastaje značajna količina nusproizvoda poznatog kao jabučna komina, koja ima potencijalnu nutritivnu vrijednost zbog prisutnosti mnogih hranjivih tvari, među kojima su i polifenoli, poznati po svojim antioksidacijskim svojstvima. Upravo zbog svog nutritivnog sastava, jabučna komina nalazi svoju primjenu u brojnim granama industrije, od prehrambene do farmaceutske. Iz tog razloga, cilj ovog rada je utvrđivanje primjene korisnih polifenola jabučne komine u svakodnevici te kvalitativna analiza polifenolnih spojeva u svrhu njezine daljnje oporabe. Istraživanje se provodilo na četiri različite sorte jabuka, a količine hranjivih tvari u jabučnoj komini dokazane su pomoću HPLC analize pojedinačnih fenolnih spojeva, te ukupnih fenolnih, flavonoidnih i neflavonoidnih spojeva. Navedeni rezultati dodatno su potkrijepljeni antioksidacijskim testovima metodama ABTS, FRAP i DPPH. Istraživanje ukazuje na veliki potencijal jabučne komine kao izvora prirodnih polifenola s primjenom u različitim industrijama. Rad pridonosi razumijevanju nutritivne vrijednosti jabučne komine i potencijala njenog korištenja u proizvodnji dodataka prehrani i drugih proizvoda. Rezultati istraživanja mogu poslužiti kao temelj za daljnje studije i komercijalne aplikacije polifenola dobivenih iz jabučne komine.

Abstract (english)

The apple is one of the most widespread fruit species in the world, used both for fresh consumption and for the production of various processed products. The processing of apples produces a significant amount of byproduct known as apple pomace, which has potential nutritional value due to the presence of many nutrients, including polyphenols, known for their antioxidant properties. Due to its nutritional composition, apple pomace is used in numerous industries, from food to pharmaceuticals. For this reason, the aim of this study is to determine the application of the beneficial polyphenols in apple pomace in daily life and to perform a qualitative analysis of the polyphenolic compounds for further use.
The study was carried out on four different apple varieties and the amounts of nutrients in apple pomace were determined by HPLC analysis of the individual phenolic compounds as well as the total phenolic, flavonoid and non-flavonoid compounds. These results were also confirmed by antioxidant tests using the ABTS, FRAP and DPPH methods. The study shows the great potential of apple pomace as a source of natural polyphenols with applications in various industries. The study contributes to the understanding of the nutritional value of apple pomace and its potential use in the production of food supplements and other products. The research results can serve as a basis for further studies and commercial applications of polyphenols from apple pomace.

• Keywords: jabuka; komina; oporaba; polifenolni spojevi; antioksidacijska aktivnost
• Keywords (english): apple; pomace; utilisation; polyphenolic compounds; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:149:544872
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Advanced materials and technologies; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-18 11:47:14