Razvoj i validacija HPLC-DAD metode za određivanje smjese odabranih ksenobiotika

master's thesis

• Title (english): Development and validation of the HPLC-DAD method for thedetermination of a mixture of selected xenobiotics
• Author: Anamaria Maslač
• Mentor: Danijela Ašperger (mentor)
• Committee member: Danijela Ašperger (predsjednik povjerenstva)
• Committee member: Davor Dolar (član povjerenstva)
• Committee member: Kristina Tolić Čop (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Ksenobiotici u okolišu završavaju najvećim djelom zbog antropološkog djelovanja i to osobito zbog neučinkovite obrade otpadnih voda iz industrijskih postrojenja. Praćenje ksenobiotika moguće je jedino analitičkim metodama, a jedna od najučinkovitijih je tekućinska kromatografija visoke učinkovitosti s detektorom s nizom dioda (HPLC-DAD). Da bi bilo moguće određivati ksenobiotike različitih fizikalno-kemijskih svojstava istovremeno nužno je izabrati odgovarajuću nepokretnu i pokretnu fazu za kromatografsku separaciju izabranih ksenobiotika. Nakon razvoja metode i odabira kromatografskih uvjeta metodu je nužno validirati. To podrazumijeva određivanje niza validacijskih parametara. Dakle, glavni ciljevi ovog rada su razvoj kromatografske metode i određivanje validacijskih parametara
HPLC-DAD metode te potvrditi njenu primjenu za analizu izabranih ksenobiotika u uzorcima.

Abstract (english)

Xenobiotics in the environment mostly end up thanks to anthropological action, especially due to inefficient treatment of waste water from industrial plants. Monitoring of xenobiotics is only possible with analytical methods, and one of the most effective is highperformance liquid chromatography with a diode array detector (HPLC-DAD). In order to be able to determine xenobiotics with different physico-chemical properties at the same time, it is necessary to choose an appropriate stationary and mobile phase for the chromatographic separation of the selected xenobiotics. After developing the method and choosing the chromatographic conditions, it is necessary to validate the method. This implies the determination of a number of validation parameters. Therefore, the main goals of this work are the development of the chromatographic method and the determination of the validation parameters of the
HPLC-DAD method and to confirm its application for the analysis of selected xenobiotics in samples.

• Keywords: ksenobiotici; pesticidi; antiparazitci; validacija; HPLC-DAD
• Keywords (english): xenobiotics; pesticides; antiparasitcs; validation; HPLC-DAD
• Language: croatian
• URN:NBN: urn:nbn:hr:149:891927
• Project: Number: MYP-G6087; Title: Oporaba vode i membranski separacijski procesi za pouzdanu i održivu opskrbu vodom; Title: Water Reuse and Membrane Separation Processes for a Reliable and Sustainable Water Supply; Acronym: WaRMem; Leader: Davor Dolar; Jurisdiction: Belgium; Funder: Organizacija Sjevernoatlantskog sporazuma; Funding stream: Science for Peace and Security
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra primijenjene kemije (sveučilišni/a magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-01
• Created on: 2024-11-07 10:33:21