Priprava i karakterizacija kompozita na temelju kitozana i biokeramike kao sustava za dostavu lijeka

master's thesis

• Title (english): Preparation and characterization of composite based on chitosan and bioceramics as drug delivery systems
• Author: Ines Ljubičić
• Mentor: Anamarija Rogina (mentor)
• Committee member: Anamarija Rogina (predsjednik povjerenstva)
• Committee member: Iva Bazina (član povjerenstva)
• Committee member: Andreja Žužić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Inženjerstvo tkiva je interdisciplinarno područje, koje primjenjuje teorijska i inženjerska znanja za razvoj biomaterijala koji obnavljaju, održavaju ili poboljšavaju funkciju tkiva. Jedan od izazova u ovom području je razvoj biomimetičkih okosnica za obnovu koštanog tkiva, u koje se također mogu ugraditi lijekovi, faktori rasta ili druge biološke komponente koje bi pospješile regenerativni učinak. Materijali koji se primjenjuju za takve slučajeve moraju biti kompatibilni s tkivom i ne smiju izazivati imunosne reakcije, stoga su u inženjerstvu koštanog tkiva popularan izbor kitozan i hidroksiapatit, koji se po sastavu podudaraju s organskim i anorganskim dijelom kosti. Kitozan je linearni polimer koji posjeduje svojstva kao što su biorazgradljivost, biokompatibilnost, hemostatična, protuupalna i antibakterijska svojstva, koja mogu potaknuti regenerativne procese u organizmu, dok dodatak hidroksiapatita omogućuje interakciju materijala s kosti. Cilj ovog rada je priprava kompozitnih okosnica na temelju kitozana i hidroksiapatita kao potencijalnih nosača antitumorskog lijeka, doksorubicina, pri liječenju koštanih defekata nastalih uklanjanjem tumorskog tkiva. Kompozitne okosnice pripravljene su metodom toplinski inducirane fazne separacije uz naknadnu liofilizaciju. Dobivene kompozitne okosnice identificirane su i karakterizirane uz pomoć infracrvene spektroskopske analize s Fourierovom transformacijom uz prigušenu totalnu refleksiju (ATR-FTIR), pretražne elektronske mikroskopije uz energijski razlučujuću rendgensku analizu (SEM-EDX) i fluorescencijske spektroskopije. Ispitan je utjecaj biokeramike na sastav, mikrostrukturu, stupanj apsorpcije vode, bioaktivnost i otpuštanje lijeka doksorubicina.

Abstract (english)

Tissue engineering is an interdisciplinary field that applies scientific and engineering knowledge to develop biomaterials that regenerate, maintain or improve tissue function.
One of the challenges in this field is the development of biomimetic scaffolds for the regeneration of bone tissue which can also incorporate drugs, growth factors or other biological components that would enhance the regenerative effect. The materials used for such cases must be compatible with the tissue and must not cause immunogenic reactions, therefore chitosan and hydroxyapatite, which in composition mimic the organic and inorganic part of the bone, are popular choices in bone tissue engineering. Chitosan is a linear polymer that has properties such as biodegradability, biocompatibility, hemostatic, anti-inflammatory and antibacterial properties, which can stimulate regenerative processes in the body, while the addition of hydroxyapatite enables the material to interact with the inorganic phase of the bone. The goal of this work is the preparation of composite scaffolds based on chitosan and hydroxyapatite as carriers of the antitumor drug, doxorubicin, in the treatment of bone defects caused by the removal of tumor tissue. Composite scaffolds were prepared by the thermally induced phase separation with subsequent lyophilization. The resulting composite scaffolds were identified and characterized using Fourier transform infrared spectroscopic analysis with attenuated total reflection (ATR-FTIR), scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDX) and fluorescence spectroscopy. The influence of bioceramics on the composition, microstructure, water absorption, bioactivity and drug release of doxorubicin was studied.

• Keywords: inženjerstvo tkiva; kitozan; hidroksiapatit; okosnice; doksorubicin
• Keywords (english): bone tissue engineering; chitosan; hydroxyapatite; scaffold; doxorubicin
• Language: croatian
• URN:NBN: urn:nbn:hr:149:772196
• Project: Number: UIP-2020-02-6201; Title: Pametni sustavi za dostavu lijeka pri liječenju tumora kosti; Title: Smart drug delivery systems in bone tumour treatment; Acronym: SmartCar; Leader: Anamarija Rogina; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Installation Research Projects
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-12-31
• Created on: 2024-11-11 14:12:40