Kolagenska prevlaka za bioaktivaciju površine titanijevog implantata

master's thesis

• Title (english): Collagen coating for bioactivation of the titanium implant surface
• Author: Martina Filipović
• Mentor: Jozefina Katić (mentor)
• Mentor: Željka Petrović (komentor)
• Committee member: Jozefina Katić (predsjednik povjerenstva)
• Committee member: Helena Otmačić Ćurković (član povjerenstva)
• Committee member: Željka Petrović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Metalni materijali, posebice titanij i njegove legure, pripadaju grupi najčešće korištenih materijala za izradu implantata. Iako posjeduju dobra mehanička i biokompatibilna svojstva, ubrajaju se u bioinertne materijale. Ugradnja titanijevog implantata u ljudsko tijelo ne potiče značajne biološke reakcije, tj. stanične procese potrebne za interakciju površine implantata s biološkim tkivima i poticanje rasta tkiva, odnosno oseointegraciju. Stoga je u ovom radu provedena funkcionalizacija površine titanija prevlakom kolagena, koji je ključni strukturni protein s važnom ulogom u strukturi kostiju i regeneraciji tkiva. Funkcionalizacija kolagenskom prevlakom provedena je s ciljem bioaktivacije površine titanijevog implantata, u svrhu poboljšanja njegove biokompatibilnosti, osteokonduktivnosti i oseointegracije. Nemodificirani uzorci titanija, sa spontano formiranim oksidnim slojem titanijevog (IV) oksida, i uzorci modificirani kolagenskom prevlakom, uronjeni su u otopinu Fusayama umjetne sline tijekom 16 tjedana. Bioaktivnost kolagenske prevlake utvrđena je promatranjem spontanog formiranja depozita kalcijevih fosfata na površini uzoraka uslijed urona. Morfologija, mikrostruktura i kemijski sastav supstrata prije i nakon uranjanja u otopinu umjetne sline karakterizirani su pretražnom elektronskom mikroskopijom (SEM), energijski razlučujućom rendgenskom spektrometrijom (EDS) i infracrvenom spektroskopijom s Fourierovom transformacijom uz prigušenu totalnu refleksiju (ATR-FTIR). Elektrokemijsko ponašanje uzoraka ispitano je metodom elektrokemijske impedancijske spektroskopije (EIS) u otopini umjetne sline koja simulira stvarne uvjete primjene implantata.

Abstract (english)

Metal materials, especially titanium and its alloys, belong to a group of the most commonly used materials for producing implants. Even though they possess good mechanical and biocompatible properties, they are referred to as bioinert materials. The implantation of a titanium implant into the human body does not encourage significant biological reactions, such as the cellular processes needed for interaction between the surface of the implant and biological tissue and for stimulating the growth of tissue, known as osseointegration. Therefore, in this work, the functionalization of the titanium surface with a collagen coating was conducted. Collagen is an essential structural protein with a major role in bone construction and tissue regeneration. The functionalization with the collagen coating was carried out with the purpose of bioactivating the titanium implant surface to improve its biocompatibility, osteoconductivity, and osseointegration. Unmodified titanium samples, with a spontaneously formed oxide layer of titanium (IV) oxide, and samples modified with a collagen coating were immersed in Fusayama artificial saliva solution for 16 weeks.
The bioactivity of the collagen coating was established by monitoring the spontaneous formation of calcium phosphate deposits on the sample surface after immersion. Morphology, microstructure, and chemical composition of the substrate before and after immersion into the artificial saliva solution were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and attenuated total reflectance using Fourier transform infrared spectroscopy (ATR-FTIR). The electrochemical behavior of the samples was examined using electrochemical impedance spectroscopy (EIS) in artificial saliva solution, which simulates real-life conditions of implant application.

• Keywords: titanijevi implantati; kolagenska prevlaka; oseointegracija; elektrokemijska impedancijska spektroskopija (EIS); SEM/EDS analiza; ATR-FTIR spektroskopija; korozijska otpornost
• Keywords (english): titanium implants; collagen coating; osseointegration; electrochemical impedance spectroscopy (EIS); SEM/EDS analysis; ATR-FTIR spectroscopy; corrosion resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:149:559994
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2031-10-01
• Created on: 2024-11-11 14:35:55