Priprava okosnica na temelju polisaharida kao nosača lijeka

undergraduate thesis

• Title (english): Preparation of polysaccharide-based scaffolds as drug carriers
• Author: Robert Bačani
• Mentor: Anamarija Rogina (mentor)
• Committee member: Anamarija Rogina (predsjednik povjerenstva)
• Committee member: Iva Bazina (član povjerenstva)
• Committee member: Marica Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Bioragradljive okosnice česta su tema današnjih istraživanja u području tkivnog inženjerstva i biomedicinske primjene. Uz svoja intrinzična svojstva biokompatibilnosti, biorazgradljivosti i netoksičnosti, pružaju optimalnu podlogu za daljnje modifikacije, bilo za svrhu obnove oštećenog tkiva, djelovanja kao privremene potpore ili čak lokalizirane dostave lijekova. Također, razni načini priprave samog materijala omogućuju stvaranje željene strukture koja je namijenjena za ciljanu svrhu. Cilj ovog rada je priprava kitozanskih okosnica modificiranih kalcij-fosfatnom keramikom (hidroksiapatitom) kao potencijalnih nosača antitumorskog lijeka (doksorubicina) uz istovremeno poticanje obnove tkiva Pripremljene su kompozitne okosnice s različitim udjelom hidroksiapatita (0, 10, 20 i 30%). Provedeni su postupci toplinski inducirane fazne separacije i liofilizacije za izradu okosnica. Time se omogućuje stvaranje poroznih struktura unutar samog materijala čime se povećava specifična površina okosnica. Dobivene strukture identificirane su i karakterizirane energijski razlučujućom rendgenskom spektroskopijom (eng. Energy-Dispersive X-ray Spectroscopy, EDS), pretražnom elektronskom mikroskopijom (eng. Scanning Electron Microscopy, SEM) i infracrvenom spektroskopijom s Fourierovom transformacijom (eng. Fourier Transform Infrared Spectroscopy, FTIR) što je ukazalo na ugradnju kalcij-fosfatne faze u polimernu matricu kitozana. Dodatno, pretražna elektronska mikroskopija je potvrdila poroznu strukturu okosnica s međusobno povezanim porama. Kapacitet apsorpcije vode okosnica istražen je u fosfatnom puferu tijekom 24 sata. Kompozitne okosnice pokazale su manji kapacitet apsorpcije vode nego okosnice čistog kitozana. Otpuštanje lijeka doksorubicina praćeno je u fosfatnom puferu koristeći fluorescencijsku metodu tijekom 24 sata. Utvrđeno je da sustavi s manjim udjelom hidroksiapatita pokazuju naglo početno otpuštanje lijeka.

Abstract (english)

Biodegradable scaffolds are a frequent topic of today's research in the field of tissue engineering and biomedical applications. With their intrinsic properties of biocompatibility, biodegradability and non-toxicity, they provide an optimal basis for further modifications, either for the purpose of regenerating damaged tissue, acting as temporary support or even localized drug delivery. Also, different ways of preparing the material itself enable the creation of the desired structure that is intended for the target purpose. The aim of this work was to prepare the chitosan scaffolds modified with calcium-phosphate ceramics (hydroxyapatite) as potential carriers of an antitumor drug (doxorubicin) with simultaneous tissue regeneration. Composite scaffolds with different amounts of hydroxyapatite (0, 10, 20 and 30%) were prepared. The thermally induced phase separation and lyophilization were carried out for the production of scaffolds. This enables the creation of porous structures, which increases the specific surface area of the scaffolds. The resulting structures were identified and characterized by Energy-Dispersive X-ray Spectroscopy (EDS), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) which indicated the incorporation of the calcium-phosphate phase into the polymer matrix. Additionally, SEM indicated the porous structure with interconnected pores. The water absorption capacity of the scaffolds was investigated in a phosphate buffer for 24 hours. Composite scaffolds showed lower absorption and swelling capacity than pure chitosan scaffolds. The release of the drug doxorubicin was monitored in a phosphate buffer using the fluorescence method for 24 hours. It was determined that the release of doxorubicin from composite scaffolds was initially higher for composite scaffolds with lower amounts of hydroxyapatite.

• Keywords: okosnica; kitozan; hidroksiapatit; doksorubicin; otpuštanje lijeka
• Keywords (english): scaffold; chitosan; hydroxyapatite; doxorubicin; drug release
• Language: croatian
• URN:NBN: urn:nbn:hr:149:936111
• Project: Number: UIP-2020-02-6201; Title: Pametni sustavi za dostavu lijeka pri liječenju tumora kosti; Title: Smart drug delivery systems in bone tumour treatment; Acronym: SmartCar; Leader: Anamarija Rogina; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Installation Research Projects
• Study programme: Title: Materials Science and Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-06-01
• Created on: 2024-11-17 17:41:34