Utjecaj matrice uzorka na kvantitativno kromatografsko određivanje N-nitrozoamina

master's thesis

• Title (english): Influence of matrix composition on quantitative chromatographic determination of N-nitrosoamines
• Author: Nensi Tomas
• Mentor: Sandra Babić (mentor)
• Committee member: Sandra Babić (predsjednik povjerenstva)
• Committee member: Krešimir Košutić (član povjerenstva)
• Committee member: Dragana Mutavdžić Pavlović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Danas je čista voda jedan od najvrjednijih resursa na svijetu. Za osiguravanje zdravstveno ispravne vode provode se različiti postupci obrade vode, a neizostavan korak pri tome je dezinfekcija vode. Postupak dezinfekcije najčešće se provodi upotrebom klora. Primarni cilj dezinfekcije je uništavanje patogena, ali pritom može doći do nastanka dezinfekcijskih nusprodukata koji štetno utječu na ljudsko zdravlje. N-nitrozamini su spojevi koji spadaju u dezinfekcijske nusprodukte, a sve više pažnje privlače zbog svoje toksičnosti i kancerogenog djelovanja pri niskim koncentracijama. U vodi, N-nitrozamini, najviše nastaju prilikom upotrebe kloramina kao dezinfekcijskog sredstva. N-nitrozamini se u okolišu nalaze u vrlo niskim koncentracijama pa je cilj ovog rada ispitati utjecaj matrice uzorka na kvantitativno kromatografsko određivanje N-nitrozamina. U radu su korišteni uzorci sintetske bazenske, sintetske bočate i vodovodne vode. Ispitivanja su provedena sa smjesom sedam
N-nitrozamina: N, N-dibutilnitrozni amid (NDBA), N, N-dietilnitrozni amid (NDEA),
N, N-dimetilnitrozni amid (NDMA), N,N-dipropilnitrozni amid (NDPA), 4-nitrozomorfolin (NMOR), 1-nitrozopiperidin (NPIP) i 1-nitrozopirolidin (NPYR). Svi uzorci analizirani su tekućinskom kromatografijom visoke djelotvornosti s detektorom s nizom dioda (HPLC-DAD), a prije same HPLC analize uzorci su pripremljeni primjenom postupka ekstrakcije čvrstom fazom (SPE).
Za sve matrice određena je učinkovitost ekstrakcije čvrstom fazom, ponovljivost te linearno područje metode.

Abstract (english)

Today, clean water is one of the most valuable resources in the world. Various water treatment processes are implemented to ensure safe drinking water and disinfection is an essential step in this process. The disinfection procedure is most commonly carried out using chlorine. The primary goal of disinfection is to eliminate pathogens, but during this process, disinfection by-products that adversely affect human health may be formed. N-nitrosamines are compounds that fall into the category of disinfection by-products, attracting increasing attention due to their toxicity and carcinogenic effects at low concentrations. In water,
N-nitrosamines primarily form during the use of chloramines as disinfectants. N-nitrosamines are found in the environment in very low concentrations and the aim of this study is to examine the influence of matrix composition on the quantitative chromatographic determination of N-nitrosamines. The work utilized samples of synthetic pool water, synthetic brackish water and tap water. The investigations were conducted on a mixture of seven
N-nitrosamines: N, N-dibutylnitrous amide (NDBA), N, N-diethylnitrous amide (NDEA),
N, N-dimethylnitrous amide (NDMA), N, N-dipropylnitrous amide (NDPA), 4-nitrosomorpholine (NMOR), 1-nitrosopiperidine (NPIP) and 1-nitrosopyrrolidine (NPYR). All samples were analyzed using high-performance liquid chromatography with a diode array detector
(HPLC-DAD). Prior to the HPLC analysis, samples were prepared using the solid-phase extraction (SPE) method. The efficiency of solid-phase extraction was determined for all matrices, and the repeatability and linearity of the method were determined.

• Keywords: dezinfekcija vode; dezinfekcijski nusprodukti; N-nitrozamini; ekstrakcija čvrstom fazom; tekućinska kromatografija visoke djelotvornosti
• Keywords (english): water disinfection; disinfection by-products; N-nitrosamines; solid phase extraction; high-performance liquid chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:149:541058
• Study programme: Title: Materials Science and Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-11-29
• Created on: 2024-12-02 11:56:53