Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva

undergraduate thesis

• Title (english): Biological evaluation methods of materials suitable for artificial tissues
• Author: Maja Perčić
• Mentor: Marica Ivanković (mentor)
• Committee member: Marica Ivanković (predsjednik povjerenstva)
• Committee member: Jelena Macan (član povjerenstva)
• Committee member: Hrvoje Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2015-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Basic Technical Sciences; Materials

Abstract

Strategija inženjerstva tkiva oslanja se na kompleksne interakcije stanica i biomaterijala (nosača) kako bi se ostvarila uspješna regeneracija oštećenog tkiva. U tu svrhu biomaterijali su podvrgnuti ispitivanjima, naročito onima koja se odnose na procjenu biokompatibilnosti, odnosno sposobnosti materijala za obavljanje svoje terapeutske funkcije bez izazivanja nepoželjnih (upalnih) reakcija u tijelu pacijenta. In vitro testiranje je pokušaj simuliranja in vivo okruženja u strogo kontroliranim uvjetima.
U ovom radu, dan je literaturni pregled izdvojenih bioloških metoda koje se upotrebljavaju za ispitivanje pogodnosti primjene materijala u inženjerstvu koštanog tkiva. Kolorimetrijske metode najčešće su korištene metode za brze testove citotoksičnosti materijala. Testovi kojima se utvrđuje umnažanje (proliferacija) i razvoj (diferencijacija) stanica temelje se na kvantitativnom određivanju specifičnih proteina (osteogenih markera), imunološkim fluorescentnim metodama bojanja te na histološkom snimanju biomaterijala.

Abstract (english)

Bone tissue engineering strategy relies on the complex interactions between cells and biomaterials (scaffolds) to achieve a successful regeneration of the bone tissue. For this purpose, biomaterials undergo testing, especially those for the assessment of material‘s biocompatibility, i.e. the ability of biomaterial to perform its desired therapeutically function, without eliciting any undesirable response. In vitro testing is an attempt to simulate the in vivo situation in a strictly controlled environment.
In this work, several biological methods that are used for materials assessment as candidate in tissue engineering have been described. Colorimetric methods are mostly used for quick cytotoxicity assays of materials. Tests that determine the cell proliferation and differentiation are based on quantification of specific proteins (osteogenic markers), immunological fluorescence staining methods, and on histological evaluation of biomaterials.

• Keywords: inženjerstvo tkiva; 3D nosači; in vitro testiranje
• Keywords (english): tissue engineering; 3D scaffolds; in vitro testing
• Language: croatian
• URN:NBN: urn:nbn:hr:149:527649
• Study programme: Title: Chemical Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-09-19 12:40:25