Nedestruktivne metode analize slitina olova

undergraduate thesis

• Title (english): Non destructive methode of analysis lead alloy
• Author: Mia Marić
• Mentor: Danijela Ašperger (mentor)
• Committee member: Danijela Ašperger (predsjednik povjerenstva)
• Committee member: Mirta Čizmić (član povjerenstva)
• Committee member: Lidija Furač (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2017-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Analytic Chemistry

Abstract

Tankoslojna kromatografija je brza i učinkovita analitička metoda za odvajanje i detekciju brojnih metalnih kationa. Radi svoje jednostavnosti i niskih troškova jedna je od najčešće korištenih kromatografskih tehnika. Sastoji se od nekoliko koraka koji uključuju nanošenje uzoraka na kromatografsku podlogu, razvijanje kromatograma te vizualizacija komponenata uzorka. Detekcijske metode mogu biti destruktivne i nedestruktivne, a temelje se na kemijskim i fizikalnim načelima. Vizualizacija se najčešće provodi reakcijom između reagensa i komponenata uzorka pod dnevnim ili ultraljubičastim svijetlom. Prskanje je najčešći način kojim se vrši detekcija. Tankoslojna kromatografija je odabrana kao metoda za analizu slitine povijesne važnosti koja je prethodno anodno uzorkovana. Kako je olovo glavni sastojak slitine, bilo je potrebno pronaći odgovarajući reagens ili kombinaciju reagensa kojim će se Pb2+ moći detektirati. Sedam metalnih kationa (Cu2+, Sn2+, Zn2+, Pb2+, Fe3+, Ni2+, Al3+) i njihova smjesa su kromatografirani na tankom sloju mikrokristalinične celulozne podloge uz pokretnu fazu koja se sastoji od 30 mL smjese otapala čiji je omjer V(n-butanol) : V(H2O) : V(HCl) = 60:5:35, v/v/v metodom tankoslojne kromatografije. Provedenim „spot“ testom je odabrano pet kombinacija reagensa za izvođenje detekcije metalnih kationa. Dobiveni kromatogrami su razmatrani u odnosu na boju mrlje kationa i boju podloge. Potrebno je da boja podloge kromatograma bude što svjetlija, a boja mrlje tamnija kako bi se postigao zadovoljavajući kontrast za bolju vizualizaciju.

Abstract (english)

Thin layer chromatography (TLC) is fast and efficient analitical methode for determination and seperation of many metal cations. Because of its simplificity and low cost thin layer chromatogpraphy is one of the most used chromatographic techniques. It consist of several steps that include application sample to a chromatografic plate, chromatogram development, and visualisation of the components in sample. Detection method can be destructive or nondestructive and it is based on physical or chemical principles. Visualization is usually done under daylight or ultraviolet light after the reaction between reagenr and components of the sample. Spraying is the most commonly used method for detection. TLC is selected method for analyzing alloys of historical significance which was preceded by anodic sampling. Because lead is main component of alloys it was important to find ideal reagent or combination of reagent that may detect Pb2+. Seven metal cations (Cu2+, Sn2+, Zn2+, Pb2+, Fe3+, Ni2+, Al3+) and their mixture have been chromatographed on microcrystalline cellulose with 30 mL of mobile phase V(n-butanol) : V(H2O) : V(HCl) = 60:5:35, v/v/v. Spot test is used to chose five combination of reagent that will use for detection of metal cations. Chromatogram were analyzed by colour of spot and background colour of chromatogram. To achive apropriate contrast between spot colour and background colour it is important that stain is darker than background of chromatogram.

• Keywords: tankoslojna kromatografija; olovo; detekcija; reagensi za vizualizaciju
• Keywords (english): thin-layer chromatography; lead; detection; visualization reagents
• Language: croatian
• URN:NBN: urn:nbn:hr:149:739605
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Applied organic chemistry; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-13 09:12:47