Priprava i karakterizacija materijala na temelju kitozana i borne kiseline

undergraduate thesis

• Title (english): Preparation and characterisation of materials based on chitosan and boric acid
• Author: Donna Danijela Dragun
• Mentor: Anamarija Rogina (mentor)
• Committee member: Anamarija Rogina (predsjednik povjerenstva)
• Committee member: Hrvoje Ivanković (član povjerenstva)
• Committee member: Leonard Bauer (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Kitozan je prirodni polimer dobiven deacetilacijom hitina, odličnih biokompatibilnih svojstava zahvaljujući -NH2 i -OH funkcionalnim skupinama. Posjeduje polikationsku prirodu, koja omogućuje interakcije s raznim biomolekulama kao što su DNK, proteini i lipidi. Ovisno o stupnju deacetilacije i načinu pripreme, postoje različita svojstva i stupnjevi čistoće. Pogodna svojstva čine kitozan korisnim u nizu industrijskih primjena među kojima su primjena u medicini, farmaciji, agronomiji, kozmetici, tkivnom inženjerstvu i prehrambenoj industriji. Važna biološka svojstva kitozana su biorazgradljivost, biokompatibilnost, antimikrobno, hemostatično, fungistatično i protuupalno djelovanje te sposobnost mukoadhezije, koja mogu potaknuti zacjeljivanje rana, obnovu hrskavičnog te koštanog tkiva i slično. Kitozan je polisaharid topljiv u razrijeđenim otopinama kiselina što omogućuje pripravu materijala različitih oblika ovisno o njihovoj namjeni, poput filmova, vlakana, mikročestica ili poroznih struktura. Cilj ovog rada je priprema, identifikacija i karakterizacija filmova na temelju kitozana modificiranog bornom kiselinom u svrhu poboljšanja bioloških svojstava kitozana. Filmovi su pripremljeni metodom izlijevanja i sušenja, dok su identifikacija i karakterizacija provedene uz pomoć infracrvene spektroskopske analize s Fourierovim transformacijama (FTIR), rendgenske difrakcijske analize (XRD) i pretražne elektronske mikroskopije s energetski disperzivnom rendgenskom spektroskopijom (SEM/EDS). Istražen je utjecaj različitog stupnja deacetilacije kitozana (85 i 95 %) te vrsta otapala (aceton i natrijev hidroksid) tijekom priprave na sastav filmova.

Abstract (english)

Chitosan is a natural polymer obtained by deacetylation of chitin, with excellent biocompatible properties thanks to -NH2 and -OH functional groups. It has a polycationic nature, which enables interactions with various biomolecules such as DNA, proteins and lipids. Depending on the degree of deacetylation and the method of preparation, there are different properties and degrees of purity. The favorable properties make chitosan useful in a number of industrial applications, including applications in medicine, pharmacy, agronomy, cosmetics, tissue engineering and the food industry. Important biological properties of chitosan are biodegradability, biocompatibility, antimicrobial, hemostatic, fungistatic and anti-inflammatory action and the ability of mucoadhesion, which can promote wound healing, restoration of cartilage and bone tissue. Chitosan is a polysaccharide soluble in diluted acid solutions, which enables the preparation of materials of different shapes depending on their purpose, such as films, fibers, microparticles or porous structures. The aim of this work is the preparation, identification and characterization of films based on chitosan modified with boric acid in order to improve the biological properties of chitosan. The films were prepared by the casting and drying method, while identification and characterization was carried out with the help of Fourier transform infrared spectroscopic analysis (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDS). The influence of different degrees of deacetylation of chitosan (85 and 95 %) and types of solvents (acetone, sodium hydroxide) during preparation on the composition of the films was investigated.

• Keywords: kitozan; filmovi; borna kiselina; identifikacija
• Keywords (english): chitosan; films; boric acid; identification
• Language: croatian
• URN:NBN: urn:nbn:hr:149:296281
• Project: Number: UIP-2020-02-6201; Title: Pametni sustavi za dostavu lijeka pri liječenju tumora kosti; Title: Smart drug delivery systems in bone tumour treatment; Acronym: SmartCar; Leader: Anamarija Rogina; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: UIP
• Study programme: Title: Chemical Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2023-02-22 12:48:06