Validacija metode i procjena mjerne nesigurnosti kromatografskog određivanja trimetoprima

master's thesis

• Title (english): Method validation and measurement uncertainty evaluation of chromatographic determination of trimethoprim
• Author: Ines Štrkalj
• Mentor: Sandra Babić (mentor)
• Committee member: Sandra Babić (predsjednik povjerenstva)
• Committee member: Dragana Mutavdžić Pavlović (član povjerenstva)
• Committee member: Irena Škorić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-02-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Globalna upotreba farmaceutika dovela je do njihove konstantne prisutnosti u okolišu. Njihova prisutnost čak i u niskim koncentracijama može značajno utjecati na ekološku ravnotežu te se stoga razvijaju analitičke metode koje će omogućavati njihovo praćenje u okolišu. Svaka analitička metoda mora osigurati pouzdane rezultate, a upravo to se postiže validacijom metode i procjenom mjerne nesigurnosti rezultata ispitivanja. U ovom radu validirana je metode tekućinske kromatografije povezana s detektorom s nizom dioda (HPLC-DAD) za određivanje trimetoprima. Tijekom validacije određene su izvedbene karakteristike metode (selektivnost/specifičnost, linearnost, preciznost, istinitost, područje primjene, granica detekcije, granica kvantifikacije i osjetljivost) na temelju čega je zaključeno da je metoda primjenjiva za određivanje trimetoprima u riječnoj vodi jer su sve izvedbene karakteristike zadovoljile dane kriterije. Korištenjem Ishikawinog dijagrama utvrđeni su najznačajniji doprinosi mjernoj nesigurnosti te je procijenjena proširena mjerna nesigurnost rezultata određivanja trimetoprima. Ovo istraživanje i dobiveni rezultati poslužit će kao primjer kako validirati analitičku metodu i procijeniti mjernu nesigurnost rezultata te će dati uvid u analitičku metodu koja se može koristiti u budućim istraživanjima.

Abstract (english)

The global use of pharmaceuticals has led to their constant presence in the environment. Their presence even at low concentrations can significantly affect the ecological balance. Therefore, analytical methods that will enable their monitoring in the environment are being developed. Analytical methods must provide reliable results what is achieved by method validation and evaluation of measurement uncertainty. In this work, high performance liquid chromatography with a diode array detector (HPLC-DAD) for the determination of trimethoprim was validated. During the validation, the performance characteristics of the method (selectivity/specificity, linearity, precision, accuracy, working range, limit of detection, limit of quantification and sensitivity) were determined, based on which it was concluded that the method is suitable for its purpose. Using the Ishikawa diagram, the most significant contributions to measurement uncertainty were determined and the expanded measurement uncertainty of trimethoprim determination results was estimated. This research and the obtained results will serve as an example of how to validate an analytical method and assess measurement uncertainty and will provide insight into an analytical method that can be used in future research.

• Keywords: farmaceutici; trimetoprim; validacija; tekućinska kromatografija visoke djelotvornosti; mjerna nesigurnost
• Keywords (english): pharmaceuticals; trimethoprim; method validation; high performance liquid chromatography; measurement uncertainty
• Language: croatian
• URN:NBN: urn:nbn:hr:149:697319
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Advanced materials and technologies; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2023-02-26 11:31:04