Određivanje minimalne inhibitorne koncentracije 2-aril supstituiranih derivata benzimidazola na E. coli (ATCC 25922), E. faecalis (ATCC 29212), K. pneumoniae (ATCC 27736), P. aeruginosa (ATCC 9027) i S. aureus (ATCC 25923)

undergraduate thesis

• Title (english): Determination of minimal inhibitory concentration of 2-aryl supstituted benzimidazole derivatives on E. coli (ATCC 25922), E. faecalis (ATCC 29212), K. pneumoniae (ATCC 27736), P. aeruginosa (ATCC 9027) and S. aureus (ATCC 25923)
• Author: Leona Kuzmić
• Mentor: Dajana Kučić Grgić (mentor)
• Committee member: Dajana Kučić Grgić (predsjednik povjerenstva)
• Committee member: Vesna Ocelić Bulatović (član povjerenstva)
• Committee member: Lidija Furač (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Sve veći ubrzani porast bakterijske rezistencije danas predstavlja jedan od važnijih problema s kojim se čovječanstvo susreće stoga je sve veća potreba za sintezom novih spojeva koji imaju inhibitorno djelovanje. Derivati heterocikličkih spojeva, benzimidazoli i benzotiazoli, predstavljaju potencijalno biološki aktivne agense stoga je sve veći interes za njihovu sintezu i proučavanje antimikrobnog djelovanja. U okviru ovog rada određena je minimalna inhibitorna koncentracija (MIC) 2-aril supstituiranih derivata benzimidazola na Gram pozitivne bakterije, Enterococcus faecalis (ATCC 29212) i Staphylococcus aureus (ATCC 25923), i Gram negativne bakterije, Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 27736) i Pseudomonas aeruginosa (ATCC 9027). U tu svrhu primijenjena je makrodilucijska metoda pri čemu je spektrofotometrijski određena minimalna inhibitorna koncentracija. Provedbom eksperimenta i usporedbom rezultata dolazi se do zaključka da određeni derivati benzimidazola inhibiraju rast mikroorganizama korištenih u ovom radu u određenim koncentracijama. Prema rezultatima, vidljivo je da dobro inhibitorno djelovanje ima spoj 4-(2-(4-(6-chloro-1H-benzo[d]imidazol-2-il)fenoksi)etil)morfolin prema bakteriji E.faecalis, MIC=0,25 mg/L i S.aureus, MIC=32 mg/L. Spojevi 4-(2-(4-((4-(1H-benzo[d]imidazol-2-il)fenoksi)metil)-1H-1,2,3-triazol-1-il)etil)morfolin i 2-(4-(6-chloro-1H-benzo[d]imidazol-2-il)fenoksi)-N,N-dietiletan-1-amin također su pokazali dobro inhibitorno djelovanje prema E. faecalis i S. aureus, MIC=0,25 mg/L.

Abstract (english)

One of the most important problems facing humanity today is accelerated growth of bacterial resistance, therefore there is a growing need for the synthesis of new compounds that have an inhibitory effect. Derivates of heterocyclic compounds, benzimidazoles and benzothiazoles, represent potentially biologically active agents, therefore there is a growing interest in their synthesis and study od antimicrobial activity. This work presents the minimum inhibitory concentration of 2-aryl substituted benzimidazoles on Gram positive bacteries Enterococcus faecalis (ATCC 29212) and Staphylococcus aureus (ATCC 25923) and Gram negative bacteries Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 27736) and Pseudomonas aeruginosa (ATCC 9027). For this purpose, was used macrodilution method and the minimum inhibitory concentration was determined spectrophotometrically. By conducting experiments and comparing the results, we came to the conclusion that certain benzimidazole derivates in certain concentrations inhibit the growth of microorganisms which were used in this work. According to the results, it is evident that the compound 4-(2-(4-(6-chloro-1H-benzo[d]imidazol-2-yl)phenoxy)ethyl)morpholine has a good inhibitory effect against the bacteria E. faecalis, MIC=0.25 mg/L and S. aureus, MIC=32 mg/L. Compounds 4-(2-(4-((4-(1H-benzo[d]imidazol-2-yl)phenoxy)methyl)-1H-1,2,3-triazol-1-yl)ethyl)morpholine and 2-(4-(6-chloro-1H-benzo[d]imidazol-2-yl)phenoxy)-N,N-diethylethane-1-amine also showed good inhibitory activity against E. faecalis and S. aureus, MIC=0.25 mg/L.

• Keywords: minimalna inhibitorna koncentracija; 2-aril supstituirani benzimidazoli; Enterococcus faecalis; Staphylococcus aureus; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa
• Keywords (english): minimum inhibitory concentration; 2-aryl substituted benzimidazoles; Enterococcus faecalis; Staphylococcus aureus; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa
• Language: croatian
• URN:NBN: urn:nbn:hr:149:962528
• Project: Number: IP-2018-01-4682; Title: Novi spojevi temeljeni na bioizosterima purina za ispitivanje njihovih antitumorskih i antipatogenih djelovanja; Acronym: PurBioCaPa; Leader: Silvana Raić-Malić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Environmental Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2023-03-02 08:29:16