Validacija spektrofotometrijske metode određivanja nitrata u vodi

undergraduate thesis

• Title (english): Validation of a spectrophotometric method for the determination of nitrates in water
• Author: Ivana Bandić
• Mentor: Sandra Babić (mentor)
• Committee member: Sandra Babić (predsjednik povjerenstva)
• Committee member: Irena Škorić (član povjerenstva)
• Committee member: Martina Biošić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Nitrati su prisutni u vodi, stoga je potrebno pratiti njihovu razinu u vodi za ljudsku potrošnju budući da velika koncentracija može imati negativan utjecaj na ljudsko zdravlje. Validaciju je potrebno provesti kako bi se utvrdilo je li metoda prikladna namijeni, a može biti potpuna ili djelomična, ovisno o zahtjevima korisnika i namjeni metode. U ovom radu provedena je validacija spektrofotometrijske metode za određivanja nitrata u vodi, a ispitane karakteristike su: linearnost, preciznost (ponovljivost i međupreciznost), istinitost, granica detekcije i granica kvantifikacije, radno područje i robusnost metode. Rezultati ispitivanja potvrđuju linearnost metode u određenom radnom području (0,20 – 7,0 mg/L NO3-), preciznost, istinitost te robusnost. Određene su granica detekcije i granica kvantifikacije (0,20 mg/L NO3-). Analizom realnih uzoraka ustanovljeno je da radno područje odgovara području koncentracija nitrata u realnim uzorcima vodovodne vode.

Abstract (english)

Nitrates are present in water, so it is necessary to monitor their level in water for human consumption as high concentrations can adversely affect human health. Validation should be performed to determine whether the method is appropriate for the purpose, and may be complete or partial, depending on user requirements and the method purpose. In this research, validation was performed, and the examined characteristics were: linearity, precision (repeatability and intermediate precision), trueness, limit of detection, limit of quantification, working range and robustness of the method. The test results confirmed the linearity of the method in a certain working range (0.20 – 7.0 mg/L NO3-), precision, trueness and robustness. The limit of detection and the limit of quantification were determined (0.20 mg/L NO3-). The analysis of real samples established that the working range corresponds to the range of nitrate concentrations in samples of tap water.

• Keywords: nitrati; spektrofotometrija; validacija
• Keywords (english): nitrates; spectrophotometry; validation
• Language: croatian
• URN:NBN: urn:nbn:hr:149:218935
• Study programme: Title: Materials Science and Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-09-01
• Created on: 2023-03-23 09:03:32