Optimization of methylation method for analysis of fatty acids

master's thesis

• Title (croatian): Optimizacija metode metiliranja za analizu masnih kiselina
• Author: Antoneta Bogdan
• Mentor: Tomislav Bolanča (mentor)
• Committee member: Tomislav Bolanča (predsjednik povjerenstva)
• Committee member: Matija Cvetnić (član povjerenstva)
• Committee member: Ana Lončarić Božić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Granter: University of Split; Faculty of Chemistry and Technology; Split
• Defense date and country: 2022-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

The fatty acids profile of castor oil was evaluated by gas chromatography (GC), with major focus on the optimum condition for the methylation of the fatty acids. Single, double, and triple methylation processes were studied with the heating time and concentration of obtained fatty acid methyl esters (FAMEs) observed. The result obtained showed that subsequent methylation resulted in lesser concentration of FAMEs obtained. In addition, generally for most methylation experiments carried out in this research, the results showed that the highest total yield of FAMEs was obtained by operating at 100°C for 40 minutes. The results that were obtained was compared with results in various literatures regarding the optimum conditions for highest conversion of fatty acids to methyl esters. The comparison showed that the operating temperature, time, fatty acids matrix, and methylation method influences the concentration of converted fatty acid derived from the process. Design of experiment was used in modelling the process. In addition, the analysis of variance (ANOVA) was successfully analyzed for the methylation process. At optimal conditions, R^2 obtained for the methylation of the fatty acids was 0.9702, which signifies excellent linearity. The equation that best describes the process was also obtained in terms of coded and actual factors respectively.

Abstract (croatian)

Profil masnih kiselina ricinusovog ulja evaluiran je plinskom kromatografijom (GC), s glavnim fokusom na optimalne uvjete za metiliranje masnih kiselina. Proučavani su procesi jednostrukog, dvostrukog i trostrukog metiliranja uz promatranje vremena zagrijavanja i koncentracije dobivenih metil estera masnih kiselina (FAME). Dobiveni rezultat pokazao je da je naknadno metiliranje rezultiralo manjom koncentracijom dobivenih FAME. Osim toga, općenito za većinu eksperimenata metiliranja provedenih u ovom istraživanju, rezultati su pokazali da je najveći ukupni prinos FAME dobiven radom na 100°C tijekom 40 minuta. Dobiveni rezultati uspoređeni su s različitim rezultatima u literaturi o optimalnim uvjetima za najveću konverziju masnih kiselina u metilne estere. Usporedba je pokazala da radna temperatura, vrijeme, matrica masnih kiselina i metoda metiliranja utječu na koncentraciju pretvorene masne kiseline izvedene iz procesa. U modeliranju procesa korišten je eksperimentalni dizajn. Osim toga, analiza varijance (ANOVA) uspješno je analizirana za proces metiliranja. U optimalnim uvjetima, R^2 dobiven za metiliranje masnih kiselina bio je 0,9702, što označava izvrsnu linearnost. Jednadžba koja najbolje opisuje proces također je dobivena u smislu kodiranih odnosno stvarnih faktora.

• Keywords: fatty acids; methylation; optimization; experimental design; analysis of variance (ANOVA)
• Keywords (croatian): masne kiseline; metiliranje; optimizacija; eksperimentalni dizajn; analiza varijance (ANOVA)
• Language: english
• URN:NBN: urn:nbn:hr:149:305706
• Study programme: Title: Chemical and environmental technology - graduate - university; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2023-04-05 10:11:00