Dehalogenacija 1,3-diklor-2-propanola u prisustvu halogenhidrin-dehalogenaze

master's thesis

• Title (english): 1,3-dichloro-2-propanol in the presence of halohydrin dehalogenase
• Author: Ivana Bigor
• Mentor: Zvjezdana Findrik Blažević (mentor)
• Committee member: Zvjezdana Findrik Blažević (predsjednik povjerenstva)
• Committee member: Marija Vuković Domanovac (član povjerenstva)
• Committee member: Martina Sudar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-07-05, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Enzimi su biokatalizatori koji kataliziraju reakcije u živim organizmima. Mogućnost primjene enzima izvan stanica u kojima se prirodno nalaze omogućila je korištenje enzima u industrijske svrhe. Osim niza prednosti poput, blagih reakcijskih uvjeta i netoksičnosti za okoliš, razlog njihove česte primjene je mogućnost proizvodnje optički čistih produkata što je izrazito važno u farmaceutskoj industriji. Halogenhidrin-dehalogenaza je jedan od enzima koji su našli primjenu u tom području. Moguće ga je dobiti prekomjernom ekspresijom tehnologijom rekombinantne DNA te pokazuje svojstva enantio- i regioselektivnosti u reakcijama otvaranja i zatvaranja epoksidnog prstena. Cilj ovog rada bio je provesti reakciju dehalogenacije 1,3-diklor-2-propanola kataliziranu enzimom halogenhidrin-dehalogenazom B tipa T120A. Prije reakcije je okarakteriziran enzim te određena njegova stabilnost u prisutnosti reakcijskih komponenata. Ispitana je hidroliza reakcijskih komponenata u reakcijskom mediju te utjecaj njihovih koncentracija na brzinu enzimske reakcije. Iz dobivenih rezultata procijenjeni su kinetički parametri koji opisuju matematički model enzimske reakcije. Reakcija je provedena u kotlastom reaktoru. Također, dobiveni matematički model validiran je u kotlastom reaktoru te se kao rezultat pokazalo dobro slaganje modela s eksperimentalnim podacima.

Abstract (english)

Enzymes are biocatalysts that catalyze reactions in living cells. The possibility of using enzymes outside the cells, in which they are naturally found, has enabled the use of enzymes for industrial purposes. In addition to a number of advantages such as mild reaction conditions and non-toxicity to the environment, the reason for their frequent application is the possibility of producing an optically pure products, which is extremely important in the pharmaceutical industry. Halohydrin-dehalogenase is one of the enzymes that have found application in this field. It can be obtained by overexpression by recombinant DNA technology and shows enantio- and regioselective properties in epoxy ring opening and closing reactions. The aim of this work was to carry out the dehalogenation reaction of 1,3-dichloro-2-propanol catalyzed by the enzyme halohydrin-dehalogenase B type T120A. Prior to the reaction, the enzyme was characterized and its stability in the presence of reaction components was determined. The hydrolysis of the reaction components in the reaction medium and the influence of their concentrations on the kinetics of the enzyme reaction were investigated. From the results, kinetic parameters describing the mathematical model of the enzymatic reaction were obtained. The reaction was performed in a batch reactor. Also, the obtained mathematical model was validated in a batch reactor and as a result the model showed good agreement with the experimental data.

• Keywords: biokataliza; enzimi; halogenhidrin-dehalogenaza; 1 3-diklor-2-propanol; epiklorohidrin; matematički model
• Keywords (english): biocatalysis; enzymes; halohydrin dehalogenase; 1 3-dichloro-2-propanol; epichlorohydrin; mathematical model
• Language: croatian
• URN:NBN: urn:nbn:hr:149:127338
• Project: Number: IP-2018-01-4493; Title: Enzimska sinteza fluoriranih kiralnih građevnih blokova; Acronym: EnzyFluor; Leader: Maja Majerić-Elenkov; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2023-04-06 12:56:04