Analiza pesticida kromatografskim metodama

master's thesis

• Title (english): Analysis of pesticides by chromatographic methods
• Author: Filip Čerepinko
• Mentor: Lidija Furač (mentor)
• Committee member: Lidija Furač (predsjednik povjerenstva)
• Committee member: Šime Ukić (član povjerenstva)
• Committee member: Matija Cvetnić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Pesticidi su tvari biološkog ili kemijskog podrijetla čija je osnovna svrha uništavanje organizama štetnih za biljne i životinjske proizvode. Prekomjernim korištenjem pesticida dolazi do raznih zagađenja tla, voda i sekundarnog trovanja. U ovom radu naglasak je na antikoagulantnim rodenticidima čija je upotreba povećana zbog zabrane korištenja karbofurana. Cilj rada je bio razviti i validirati kvalitativne kromatografske metode određivanja sljedećih antikoagulantnih rodenticida: brodifak, bromadiolon, difenak, difetialon, flokumafen i klorfacinon u realnim uzrocima u svrhu praćenja trovanja životinja na području Republike Hrvatske. Za svaki rodenticid razvijena je i validirana izokratska i gradijentna HPLC/DAD metoda analize, dok su za brodifak i flokumafen su razvijene LC-MS metode analize. Validacija metoda provodila se ispitivanjem parametara: granice detekcije, selektivnosti i robusnosti. Sve HPLC/DAD metode analize rodenticida su robusne i selektivne, osim izokratske metode analize difetialona koja je neselektivna. HPLC/DAD metode analize klorfacinona su specifične metode analize. LC-MS metode analize su specifične metode. Nakon ispitivanja realnih uzoraka dobiva se saznanje da 63 % realnih uzoraka nije sadržavalo tragove pesticida, dok je njih 27 % sadržavalo karbofuran, 8 % metaldehid i 2 % bromadiolon.

Abstract (english)

Pesticides are substances of biological or chemical origin whose basic purpose is to exterminate any harmful organisms for plants and animal produce. Overuse of pesticides can result in various soil and water pollution as well as secondary poisoning. In this paper, the emphasis is on anticoagulant rodenticides whose usage is increased due to ban on the use of carbofuran. The goal of this paper was to develop and validate qualitative chromatographic methods of determination of the following anticoagulant rodenticides: brodifacoum, bromadiolone, difenacoum, difethialone, flocoumafen and chlorophacinone in real samples for the purpose of monitoring animal poisoning in the territory of the Republic of Croatia. For every rodenticide it has been developed and validated an isocratic and gradient HPLC/DAD method of analysis, but only for brodifacoum and flocoumafen has been developed an LC-MS method of analysis. Method validation was carried out by examining the following parameters: limit of detection, selectivity and robustness. All HPLC/DAD methods of rodenticides analysis are robust and selective except for difethialone whos isocratic method is nonselective. All HPLC/DAD methods of analysing chlorophacinone are specific. LC-MS methods of analysis are specific. After analysing the real samples, it is known that 63 % of them didn’t contain any pesticides, 27 % carbofuran, 8 % metaldehyde and 2 % bromadiolon.

• Keywords: pesticidi; antikoagulantni rodenticidi; validacija; metoda; analiza; HPLC; LC-MS; realni uzorci
• Keywords (english): pesticides; anticoagulant rodenticides; validation; method; analysis; HPLC; LC-MS; real samples
• Language: croatian
• URN:NBN: urn:nbn:hr:149:168547
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2023-04-20 13:50:59