Potencijal okolišne bakterijske kulture u biorazgradnji ksenobiotika

master's thesis

• Title (english): Potential of environmental bacterial culture for the biodegradation of xenobiotics
• Author: Ana Šket
• Mentor: Marija Vuković Domanovac (mentor)
• Committee member: Marija Vuković Domanovac (predsjednik povjerenstva)
• Committee member: Zvjezdana Findrik Blažević (član povjerenstva)
• Committee member: Monika Šabić Runjavec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Svijet se sve više urbanizira. Rezultat sve veće urbanizacije je rast raznih industrija, poput farmaceutske. Upravo ovakvi spojevi koji se prirodno ne nalaze u okolišu, ali zbog antropogenih aktivnosti dospijevaju u njega, nazivaju se ksenobiotici. Potencijal okolišnih bakterijskih kultura za biorazgradnju ksenobiotika obećavajući je pristup u rješavanju nastalih ekoloških izazova povezanih s postojanošću kemijskih onečišćivača u okolišu. Iskorištavanjem metaboličke aktivnosti bakterijskih kultura iz okoliša, ovaj pristup nudi isplativu i učinkovitu alternativu konvencionalnim postupcima remedijacije i ima potencijal za ublažavanje negativnih učinaka ksenobiotika na zdravlje ljudi i okoliš. U ovom radu procesom biorazgradnje provedeno je uklanjanje farmaceutika amoksicilina pomoću bakterijske kulture Pseudomonas aeruginosa izolirane iz okoliša. Pokus je proveden u šaržnom reaktoru i aerobnim uvjetima u periodu od 12 dana s početnim koncentracijama amoksicilina od
278 mg L^-1 (Pa-1) i 658 mg L^-1 (Pa-2). Prema provedenim testovima osjetljivosti
P. aeruginosa je pokazala otpornost na ispitivani ksenobiotik i nastale produkte.
Rezultati istraživanja pokazuju učinkovitost procesa od 49,6 % za Pa-1, te 36,2 % za Pa-2.

Abstract (english)

The world is becoming increasingly urbanized. The result of increasing urbanization is the growth of various industries, such as the pharmaceutical industry, that produce various compounds. These compounds, which do not occur naturally in the environment but enter the environment due to anthropogenic activities, are known as xenobiotics. The potential of bacterial cultures in the environment to biodegrade xenobiotics is a promising approach to solving environmental problems related to the persistence of chemicals in the environment. By utilizing the metabolic activity of bacterial cultures in the environment, this approach offers a cost-effective and efficient alternative to remediation methods and has the potential to mitigate the harmful effects of xenobiotics on human health and the environment. In this work, the biodegradation process was used to remove amoxicillin using a bacterial culture of Pseudomonas aeruginosa isolated from the environment. The experiment was carried out in a batch reactor and under aerobic conditions for a period of 12 days with initial amoxicillin concentrations of 278 mg L^-1 (Pa-1) and 658 mg L^-1 (Pa-2). According to the sensitivity tests performed, Pseudomonas aeruginosa showed resistance to the xenobiotics tested and the resulting products. The research results showed a process efficiency of 49.6 % for Pa-1 and 36.2 % for Pa-2.

• Keywords: ksenobiotik; amoksicilin; Pseudomonas aeruginosa; biorazgradnja
• Keywords (english): xenobiotic; amoxicillin; Pseudomonas aeruginosa; biodegradation
• Language: croatian
• URN:NBN: urn:nbn:hr:149:563469
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-10-01
• Created on: 2023-07-27 10:58:42