Razvoj i validacija kromatografske metode za određivanje smjese farmaceutika u vodi

undergraduate thesis

• Title (english): Development and validation of a chromatographic method for the determination of pharmaceuticals mixture in water
• Author: Leona Čakić
• Mentor: Dragana Mutavdžić Pavlović (mentor)
• Committee member: Dragana Mutavdžić Pavlović (predsjednik povjerenstva)
• Committee member: Kristina Tolić Čop (član povjerenstva)
• Committee member: Ida Boček Pavlinac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Farmaceutici u okolišu i njihovo praćenje dva su značajna problema uzrokovana sve većim razvitkom farmaceutske industrije. Ovi spojevi u okolišu podliježu strukturnim promjenama pa tako mogu uzrokovati sinergijsko djelovanje s drugom tvari ili pak kemijski reagirati. Njihovo praćenje nužno je za pravovremeno sprječavanje ozbiljnijih posljedica. U ovom radu prikazani su i objašnjeni razvoj i validacija kromatografske tekućinske metode na HPLC-DAD uređaju u svrhu detekcije, identifikacije i kvantifikacije farmaceutika u deioniziranoj i u vodovodnoj vodi. Smjesa se sastojala od sljedećih farmaceutika: amoksicilin, atenolol prokain, ofloksacin, sulfametazin, sulfametoksazol, torasemid, karbamazepin, deksametazon, β-estradiol, diazepam i diklofenak. Razvijena je metoda gradijentna. Za nepokretnu fazu koristi
Kinetex 5 μm kolonu, a za pokretnu odabrani su voda kao anorganska i acetonitril kao organska faza. Mjerenje se provodilo pri valnim duljinama od 230, 240, 254, 275 i 290 nm, određenima snimanjem apsorpcijskih spektara farmaceutika u smjesi. Razvijena metoda validirana je eksperimentima u svrhu ispitivanja analitičkih značajki te metode, koje uključuju: linearnost metode, granice detekcije i kvantifikacije farmaceutika, osjetljivost, preciznost, istinitost, radno područje i robusnost metode. Svi eksperimenti provedeni su pomoću otopina farmaceutika u deioniziranoj i vodovodnoj vodi.

Abstract (english)

Pharmaceuticals found in water and their monitoring seem to be two common problems due to the growth of pharmaceutical industry. These compounds can often interfere and act in synergy with other substances or materials. Pharmaceutical monitoring becomes inevitable in order to prevent serious consequences beforehand. This present work deals with the development and validation of a liquid chromatographic method performed with the instrument HPLC-DAD for the detection, identification and quantification of pharmaceuticals in deionized and tap water. Mixture of pharmaceuticals used in this work includes amoxicilin, atenolol, procaine, ofloxacin, sulfamethazine, sulfametoxazole, torasemide, carbamazepine, dexamethazone, β-estradiol, diazepam and diclofenac. The developed method is gradient-based. A Kinetex 5 μm column was chosen as the stationary phase. The mobile phase consisted of water as the inorganic component and acetonitrile as the organic component. The measurement was performed under defined conditions, at wavelengths of 230, 240, 254, 275 and 290 nm, which were selected based on the absorption maximums. The developed method was validated by experiments to investigate validation parameters, such as linearity, limit of detection, limit of quantitation, sensitivity, precision, accuracy, range and robustness. All experiments were performed for pharmaceutical mixtures in both deionized and tap water.

• Keywords: kromatografija; metoda; validacija; farmaceutici; vodovodna voda
• Keywords (english): chromatography; method; validation; pharmaceuticals; tap water
• Language: croatian
• URN:NBN: urn:nbn:hr:149:715995
• Study programme: Title: Chemical Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-09-06
• Created on: 2023-09-07 10:31:09