Primjena diferencijalne pretražne kalorimetrije u karakterizaciji materijala iz biogenog izvora

undergraduate thesis

• Title (english): Application of differential scanning calorimetry in the characterization of biogenic material
• Author: Sabina Fućak
• Mentor: Gordana Matijašić (mentor)
• Committee member: Gordana Matijašić (predsjednik povjerenstva)
• Committee member: Vesna Ocelić Bulatović (član povjerenstva)
• Committee member: Krunoslav Žižek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-09-07, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Porast štetnih životnih navika uzrokuje sve veći broj jetrenih oboljenja što povećava potrebu za transplantacijom ili popravkom oštećenog tkiva. Decelularizacija prirodnih tkiva obećavajuća je metoda za dobivanje trodimenzionalne mreže za regeneraciju tkiva jer se zadržavaju prirodna struktura i komponente što predstavlja bitni čimbenik za preživljavanje stanica. Vrlo je bitno razviti metodu decelularizacije koja će dovoljno dobro ukloniti stanice, a opet sačuvati komponente izvanstanične matrice. Za decelularizaciju jetrenog tkiva korištena su dva detergenta, natrijev lauret sulfat (SLES) i natrijev lauril sulfat (SDS). U protokolima decelularizacije ispitan je utjecaj vremena izmjene medija i koncentracije detergenata te je uključena i dodatna obrada ultrazvukom u trajanju od 1, 2, 4 i 8 sati. U uzorcima su nakon decelularizacije kvantificirane biološke komponente: DNK, kolagen i glikozaminoglikani. Uzorci su nakon decelularizacije podvrgnuti digestiji te liofilizaciji. Razlikovna pretražna kalorimetrija provedena je na uzorcima nakon decelularizacije i digestije te su utvrđene vrijednosti normalizirane entalpije te početne i maksimalne temperature denaturacije. Dobiveni rezultati pokazuju da je uzorak SDS 1a, obrađen natrijevim lauril sulfatom koncentracije 1 % s vremenom izmjene medija 30 minuta, najpogodniji za daljnja ispitivanja primjene u 3D tisku. Unatoč literaturnim podacima koji ukazuju na povezanost entalpije i temperatura denaturacije s udjelom kolagena, u provedenim mjerenjima nije uočena direktna povezanost što može biti posljedica prisutnosti drugih tvari osim kolagena.

Abstract (english)

The prevalence of unfavorable lifestyle habits is leading to a growing incidence of liver disease, increasing the need for transplantation or repair of damaged tissue. Decellularization of natural tissue is a promising method to maintain a three-dimensional network for tissue regeneration, as the natural structure and components are preserved, which is an essential factor for cell survival. It is of utmost importance to develop a decellularization method that removes the cells well enough and still preserves the components of the extracellular matrix. Two detergents have been used for decellularization of liver tissue – sodium laureth sulfate (SLES) and sodium lauryl sulfate (SDS). In the decellularization protocols, the influence of medium exchange time and detergent concentration was investigated and additional ultrasound treatment was included for 1, 2, 4, and 8 hours. After decellularization, the biological components in the samples were quantified: DNA, collagen, and glycosaminoglycans. After decellularization, the samples were subjected to a digestion process and freeze-dried. After decellularization and digestion, the samples were subjected to differential scanning calorimetry and the values of normalized enthalpy and initial and maximum denaturation temperatures were determined. The results obtained show that the sample SDS 1a, treated with sodium lauryl sulfate at a concentration of 1% with a medium change of 30 minutes, is the most suitable for further testing for application in 3D printing. Despite evidence of a relationship between enthalpy and denaturation temperatures with collagen content, no direct relationship was found in the measurements, which could be due to the presence of substances other than collagen.

• Keywords: decelularizacija; natrijev lauret sulfat; natrijev lauril sulfat; izvanstanična matrica
• Keywords (english): decellularization; sodium laureth sulfate; sodium lauryl sulfate; extracellular matrix
• Language: croatian
• URN:NBN: urn:nbn:hr:149:016309
• Study programme: Title: Chemical Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-09-12 12:23:51