Određivanje fitotoksičnosti fenola, rodanida i cijanida na Allium cepa

undergraduate thesis

• Title (english): Determination of phytotoxicity of phenol, rhodanide and cyanide on Allium cepa
• Author: Lorenna Stanić
• Mentor: Dajana Kučić Grgić (mentor)
• Committee member: Dajana Kučić Grgić (predsjednik povjerenstva)
• Committee member: Martina Miloloža (član povjerenstva)
• Committee member: Lidija Furač (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-02, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Fenoli, cijanidi i rodanidi smatraju se glavnim onečišćujućim tvarima u sastavu koksne otpadne vode koja glasi jednom od najzahtjevnijih industrijskih otpadnih voda za zbrinjavanje. Onečišćujuće tvari u sastavu koksne otpadne vode izazivaju izrazito štetne fitotoksične učinke te je zato od velike važnosti istraživanje o njihovom djelovanju u svrhu procjene rizika, boljeg razumijevanja toksičnog djelovanja i razvoja metoda za zaštitu okoliša i organizama. Stoga se u ovom radu određivala fitotoksičnost fenola, rodanida i cijanida na sjemenkama luka Allium cepa pomoću testa klijavosti (eng. seed germination test) kojim se ispituje utjecaj ispitivanih tvari na klijanje i rani rast biljaka. Tijekom 21 dan sjemenke luka Allium cepa bile su u kontaktu s otopinama ispitivanim tvari, dodanih u tlo, različitih koncentracija (10, 25, 50, 75 i 100 mg/L) u kontroliranim uvjetima, podjednakim za sve tri ispitivane tvari. Tijekom trajanja eksperimenta pratio se broj izraslih izdanaka, postotak nicanja, visina izdanaka te mase svježe i suhe tvari, a rezultati su se uspoređivali s kontrolom kako bi bili sigurni da su učinci pripisani isključivo utjecaju fenola, rodanida i cijanida. Iz rezultata određen je postotak inhibicije gdje je vidljivo da najveći fitotoksični učinak na luk Allium cepa imaju cijanidi, zatim fenoli pa rodanidi.

Abstract (english)

Phenols, cyanides and rhodanides are considered to be the main pollutants in the composition of coking wastewater, which is one of the most demanding industrial wastewaters for disposing. Pollutants, in the composition of coke waste water, cause extremely harmful phytotoxic effects, which is why research of it's mechanism of action is of great importance for the purpose of risk assessment, better understanding of toxicity mechanisms and development of methods for the protection of the environment and organisms. Therefore, in this work, the phytotoxicity of phenols, rhodanides, and cyanides was determined on Allium cepa onion seeds using the seed germination test, which examines the influence of the tested substances on germination and early plant growth. During 21 days, Allium cepa onion seeds were in contact with solutions of test substances, added to the soil, in various concentrations (10, 25, 50, 75 and 100 mg/L) under controlled conditions, identical for all three tested substances. The number of germs, the germination percentage, the height of the shoots and the mass of fresh and dry matter were monitored, and the results were compared with the control to make sure that the effects were prescribed only to the influence of phenol, rhodanide and cyanide. From the results the percentage of inhibition was determined whic showed that cyanides, followed by phenols and then rhodanides have the greatest phytotoxic effect on Allium cepa seeds.

• Keywords: koksna otpadna voda; fenoli; cijanidi; rodanidi; fitotoksičnost; test klijavosti; Allium cepa
• Keywords (english): coking wastewater; phenols; cyanides; rhodanides; phytotoxicity; seed germination; Allium cepa
• Language: croatian
• URN:NBN: urn:nbn:hr:149:811627
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-03-30
• Created on: 2024-10-14 08:22:42