Development of a measurement procedure for the detection of micro and nanoplastic particles in tissue samples

master's thesis

• Title (croatian): Razvoj mjernog postupka za detekciju mikro i nanoplastičnih čestica u uzorcima tkiva
• Author: Karlo Blažević
• Mentor: Dajana Kučić Grgić (mentor)
• Committee member: Dajana Kučić Grgić (predsjednik povjerenstva)
• Committee member: Lidija Furač (član povjerenstva)
• Committee member: Martina Miloloža (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

With the mass accumulation of plastic pollution in the environment, problems are present even on the micro and nano scale. Micro and nanoplastic (MNP) particles with a diameter of less than one millimeter, mostly generated by the degradation and wearing down of larger plastic parts, are accumulating in the ecosystem and reaching the human body in different ways. The potential of the particles to adsorb harmful substances poses a big threat to ecosystems and human health. Analytical chemistry offers essential tools for the detection, quantification, and characterization of small particles, enabling researchers to study their distribution, composition, and impact. The most used methods are different types of microscopy and spectroscopy. This work provides the process of preparing and analyzing different tissue samples for MNP research. A review of the most common tissue digestion methods was made, along with two analytical methods that were used to detect, quantify, and characterize MNPs: optical microscopy and spectroscopy. MNP particles were found and identified in the tissue samples, along with present contaminants.

Abstract (croatian)

Sve većim rastom plastičnog onečišćenja u okolišu javljaju se problemi čak i u mikro i nano razmjerima. Plastične čestice promjera manjeg od milimetra koje uglavnom nastaju razgradnjom i trošenjem većih dijelova plastike, nakupljaju se u ekosustavu te različitim putevima dolaze i do organizma čovjeka. S potencijalom adsorpcije štetnih tvari, mikro- i nano plastika predstavlja veliku prijetnju ekosustavima i ljudskom zdravlju. Analitička kemija nudi bitne alate za detekciju, kvantifikaciju i karakterizaciju malih čestica, omogućujući istraživačima proučavanje njihovog sastava, distribucije i utjecaja. Najčešće metode koje se koriste su razni oblici mikroskopije i spektroskopije. Ovaj rad sadrži proces pripreme i analize različitih uzoraka tkiva za istraživanje mikro- i nano plastike. Napravljen je pregled najčešćih metoda digestije tkiva te dvije analitičke metode koje su korištene za detekciju, kvantifikaciju i karakterizaciju mikro- i nano plastike: optička mikroskopija i spektroskopija. U uzorcima tkiva pronađene su i identificirane mikro- i nanoplastične čestice, uz prisutna onečišćenja u obliku fragmenata i vlakana.

• Keywords: micro and nanoplastics; tissue; microscopy; spectroscopy
• Keywords (croatian): mikro- i nano plastika; tkivo; mikroskopija; spektroskopija
• Language: english
• URN:NBN: urn:nbn:hr:149:535951
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2025-01-01
• Created on: 2024-11-11 09:16:22