Pročišćavanje soforolipida

undergraduate thesis

• Title (english): Sophorolipids purification
• Author: Carla Alexandra Qvarnström
• Mentor: Martina Sudar (mentor)
• Committee member: Martina Sudar (predsjednik povjerenstva)
• Committee member: Goran Lukač (član povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-12, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Soforolipidi su prirodni biosurfaktanti koje sintetizira kvasac Candida koji koristi šećer i biljno ulje kao izvore nutrijenata za rast. Ovi spojevi imaju značajan potencijal za primjenu u industriji zbog svojih biorazgradivih svojstava i sposobnosti stvaranja stabilnih emulzija.
U ovom istraživanju provedena je modifikacija soforolipida u enzimski kataliziranoj reakciji te pročišćavanje dobivene reakcijske smjese. Nakon provedenog pročišćavanja svaka dobivena frakcija podvrgnuta je analizi na HPLC uređaju kako bi se precizno odredio sastav i čistoća produkata. Tijekom procesa pročišćavanja, izolirani su produkti s različitim brojem monosaharidnih jedinica, pri čemu je posebna pažnja posvećena izolaciji i analizi produkta n=1, koji predstavlja specifični cilj ovog istraživanja. Kao nusprodukti reakcije, formirani su i oligosaharidi, koji su uspješno uklonjeni iz reakcijske smjese pomoću etanola.
Ovo istraživanje pruža važne uvide u proces enzimske modifikacije soforolipida i njihovu potencijalnu primjenu u raznim industrijskim sektorima.

Abstract (english)

Sophorolipids are natural biosurfactants synthesized by the Candida yeast that uses sugar and vegetable oil as sources of nutrients for growth. These compounds have significant potential for application in industry due to their biodegradable properties and ability to form stable emulsions. In this research, the modification of sophorolipids in an enzyme-catalyzed reaction and the purification of the resulting reaction mixture was carried out.
After purification, each obtained fraction was subjected to analysis on an HPLC device in order to precisely determine the composition and purity of the product. During the purification process, products with different numbers of monosaccharide units were isolated, whereby special attention was paid to the isolation and analysis of products n=1, which represents the specific goal of this research. As byproducts of the reaction, oligosaccharides were also formed, which were successfully removed from the reaction mixture using ethanol. This research provides important insights into the process of enzymatic modification of sophorolipids and their potential application in various industrial sectors.

• Keywords: biosurfaktanti; soforolipidi; enzimska modifikacija soforolipida
• Keywords (english): biosurfactants; sophorolipids; enzymatic modification of sophorolipids
• Language: croatian
• URN:NBN: urn:nbn:hr:149:594444
• Project: Number: 101000560; Title: Rapid discovery and development of enzymes for novel and greener consumer products; Acronym: RadicalZ; Leader: Zvjezdana Findrik Blažević; Jurisdiction: eu; Funder: Europska unija; Funding stream: Horizon 2020
• Study programme: Title: Environmental Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2030-06-01
• Created on: 2024-11-14 11:22:43