Enzimska sinteza amida hidroksicimetne kiseline u vodenom mediju

undergraduate thesis

• Title (english): Enzymatic synthesis of hydroxycinnamic acid amide in aqueous medium
• Author: Laura Dimter
• Mentor: Martina Sudar (mentor)
• Committee member: Martina Sudar (predsjednik povjerenstva)
• Committee member: Zvjezdana Findrik Blažević (član povjerenstva)
• Committee member: Monika Šabić Runjavec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-12, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Biokemijske reakcije sastavni su dio svih živih bića te zahtijevaju veću brzinu od klasičnih kemijskih reakcija radi održavanja normalnog tijeka životnih funkcija. Enzimi su ključni elementi provedbe biokatalize jer povećavaju brzinu reakcija za iznos od najmanje 106 te uvelike olakšavaju sintezu i industrijsku proizvodnju tvari čije je dobivanje klasičnom kemijskom sintezom znatno teže. Zahtijevaju specifične uvjete u kojima djeluju, a prisutni su samostalni ili zadržani u cijelim stanicama i mikroorganizmima. U organizmu sudjeluju u regulaciji homeostaze radi pravilnog rada metabolizma, opskrbe energijom te tvorbe proteina, stanica i tkiva. U ovom radu proučavani su uvjeti provedbe enzimske sinteze amida hidroksicimetne kiseline u vodenom mediju s ciljem određivanja optimalnih uvjeta za provođenje željene reakcije i dobivanje željenog produkta, N-trans-feruloiltiramina.
Produkt reakcije pripada skupini amida hidroksicimetne kiseline, koji svoju ulogu pronalaze u pravilnom funkcioniranju biljaka, a primjenu u mnogim industrijama zbog privlačnih antioksidativnih, antikancerogenih i antiupalnih svojstava. Praćena je aktivnost enzima na spektrofotometru i tekućinskom kromatografu visoke djelotvornosti te je određen utjecaj
pH vrijednosti, temperature i dodatka askorbinske kiseline na aktivnost enzima.

Abstract (english)

Biochemical reactions are an integral part of all living organisms and require a higher reaction rate than classical chemical reactions to maintain the normal course of life functions. Enzymes are key elements in the implementation of biocatalysis because they increase the rate of reactions by at least 106 times and greatly facilitate the synthesis and industrial production of substances whose acquisition is significantly more difficult through classical chemical synthesis. They require specific conditions in which they can operate and are present either independently or retained in whole cells and microorganisms. In the organism, they participate in the regulation of homeostasis for the proper functioning of metabolism, energy supply and the formation of proteins, cells and tissue. In this work, the conditions for the enzymatic synthesis of hydroxycinnamic acid amide in an aqueous medium were studied with the aim of determining the optimal conditions for conducting the desired reaction and obtaining the desired product, N-trans-feruloyltyramine. The reaction product belongs to the group of hydroxycinnamic acid amides, which play a role in the proper functioning of plants and have applications in many industries due to their attractive antioxidant, anticancer and anti-inflammatory properties. The enzyme activity was monitored using a spectrophotometer and high-performance liquid chromatography. The influence of pH value, temperature and the addition of ascorbic acid on enzyme activity was determined.

• Keywords: biokataliza; N-trans-feruloiltiramin; aktivnost i stabilnost; uvjeti provedbe sinteze
• Keywords (english): biocatalysis; N-trans-feruloyltyramine; activity and stability; reaction conditions
• Language: croatian
• URN:NBN: urn:nbn:hr:149:530716
• Project: Number: 101000560; Title: Rapid discovery and development of enzymes for novel and greener consumer products; Acronym: RadicalZ; Leader: Zvjezdana Findrik Blažević; Jurisdiction: eu; Funder: Europska unija; Funding stream: Horizon 2020
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2030-06-01
• Created on: 2024-11-14 12:45:33