Aktivnost esteraze u stanicama Pseudomonas sp. u uvjetima bez i uz indukciju eritromicinom

master's thesis

• Title (english): Activity of esterase in Pseudomonas sp. cells without and with induction by erythromycin
• Author: Josip Leko
• Mentor: Zvjezdana Findrik Blažević (mentor)
• Committee member: Zvjezdana Findrik Blažević (predsjednik povjerenstva)
• Committee member: Martina Sudar (član povjerenstva)
• Committee member: Marija Vuković Domanovac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2018-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Reaction Engineering

Abstract

U ovome radu praćena je aktivnost enzima eritromicin esteraze prilikom uzgoja bakterije Pseudomonas sp. Eritromicin esteraza je enzim koji navedena bakterija sintetizira, a koji razgrađuje eritromicin; antibiotik široke primjene. Aktivnost enzima je praćena tijekom uzgoja bakterije bez dodatka eritromicina u hranjivu podlogu, s dodatkom 0,2 ppm te 200 ppm eritromicina u hranjivu podlogu. Cilj rada bio je izolirati enzim iz stanica Pseudomonas sp. uzgojenih bez, s dodatkom 0,2 ppm i dodatkom 200 ppm eritromicina u hranjivu podlogu te provesti reakciju razgradnje eritromicina.

Abstract (english)

Activity of erythromycin esterase was monitored during cultivation of Pseudomonas sp. Erythromycin esterase is an enzyme which degrades erythromycin, widely used antibiotic. Enzyme activity was monitored during microbial cultivation in the system without added erythromycin in culture medium, with added 0.2 ppm and 200 ppm of erythromycin. The aim of the work was to isolate the enzyme from Pseudomonas sp. cells cultivated without, with adding 0.2 ppm and 200 ppm of erythromycin in culture medium and to carry out the erythromycin degradation reaction with the same enzyme.

• Keywords: enzim; indukcija; aktivnost; razgradnja; eritromicin; eritromicin esteraza
• Keywords (english): enzyme; induction; activity; degradation; erythromycin; erythromycin esterase
• Language: croatian
• URN:NBN: urn:nbn:hr:149:436139
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-26 12:56:26