Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja

master's thesis

• Title (english): Interaction of model lipid membrane with flavonoids encapsulated within magnetic nanoparticles within external applied magnetic fields
• Author: Lucija Mandić
• Mentor: Helena Otmačić Ćurković (mentor)
• Committee member: Helena Otmačić Ćurković (predsjednik povjerenstva)
• Committee member: Emi Govorčin Bajsić (član povjerenstva)
• Committee member: Suzana Šegota (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2018-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Applied Chemistry

Abstract

U okviru ovog rada provedena je strukturna, morfološka i fizičko-kemijska karakterizacija magnetskih Fe3O4 mezoporoznih nanočestica sljedećim tehnikama: rendgenskom difrakcijom na polikristalnom uzorku (XRPD), pretražnom elektronskom mikroskopijom (SEM), Brunauer-Emmett-Teller (BET) analizom, UV/Vis spektrofotometrijom te infracrvenom spektroskopijom (FTIR). Provedena su ispitivanja s tri strukturno različita flavonoida (kvercetin, miricetin i miricitrin) koji su ugrađeni u Fe3O4 nanočestice. Količina ugrađenih flavonoida u nanočestice određena je spektrofotometrijskim mjerenjima apsorbancije supernatanta pomoću UV/Vis spektrofotometra. Nakon ugradnje flavonoida u mezoporozne nanočestice, praćena je kinetika njihova otpuštanja iz nanočestica u ovisnosti o eksternoj stimulaciji, tj. pri normalnim uvjetima, pod utjecajem permanentnog i oscilirajućeg magnetskog polja. Promjena strukturnih i morfoloških svojstava modelnih membrana inducirana njihovom reakcijom s flavonoidima, otpuštanim iz nanočestica pri različitim eksternim uvjetima, prije i poslije induciranog oksidativnog stresa istražena je mikroskopijom atomskih sila (AFM). Učinkovitost ugrađenih flavonoida u zaštiti modelnih membrana od oksidativnog stresa te njihova zaštitna uloga potvrđena je metodom dinamičkog raspršenja svjetlosti (DLS) te mjerenjima zeta potencijala.

Abstract (english)

The structural, morphological and physico-chemical characterizations of mesoporous magnetic Fe3O4 nanoparticles were conducted within the framework of this research using several techniques: X-ray diffraction (XRPD), scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) analysis, UV/Vis spectrophotometry and infrared spectroscopy (FTIR). The research was performed using three different flavonoids (quercetin, myricetin and myricitrin) and incorporated in Fe3O4 nanoparticles. The quantity of incorporated flavonoids in nanoparticles was determined by spectrophotometric measurment of supernatant with UV/Vis spectrophotometer. After incorporation of flavonoid within nanoparticles, their release profiles were followed by during the experiment the focus was on releasing the flavonoids from the nanoparticles at the standard conditions and in permanent and oscillating magnetic field using UV/Vis spectrophotometry. Changing the structural and morphological properties of model membranes induced by their reaction with flavonoids, laid off from the nanoparticles in different extreme conditions, before and after induced oxidative stress was investigated by atomic force microscopy. The efficiency of embedded flavonoids in protecting of model membranes from oxidative stress and their protective role was confirmed by dynamic light scattering and zeta potential measurements.

• Keywords: kvercetin; miricetin; miricitrin; Fe3O4; liposomi; oksidacijski stres
• Keywords (english): quercetin; myricetin; myricitrin; Fe3O4; liposomes; oxidative stress
• Language: croatian
• URN:NBN: urn:nbn:hr:149:060572
• Study programme: Title: Materials Science and Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-11-02 13:18:22