Priprema i karakterizacija elektroispredenih nosača za regeneraciju kože i oka

master's thesis

• Title (english): Preparation and characterization of elektrospinning scaffolds for skin and eye regeneration
• Author: Miroslav Štos
• Mentor: Emi Govorčin Bajsić (mentor)
• Committee member: Emi Govorčin Bajsić (predsjednik povjerenstva)
• Committee member: Nevenka Vrbos (član povjerenstva)
• Committee member: Igor Sutlović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2019-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Elektroispredanje je postupak proizvodnje nanovlakana pomoću elektrostatskih sila. Ljudsko tkivo sklono je oštećenju. Manje rane, ogrebotine i iščašenja, pa čak i blaži lomovi kostiju lako zarastaju. Problem su potpuno oštećeni organi koji gube funkciju, kao i veliki lomovi kostiju. U rješavanju ovog problema pomaže tkivno inženjerstvo. Tkivno inženjerstvo i proizvodnja funkcionalnih tkiva i organa dio su modernih biomedicinskih istraživanja i primjena. U tkivnom inženjerstvu stanice se zasijavaju u/na biomaterijale prije transplantacije. Ti materijali zatim služe kao privremeni nosači koji unaprjeđuju reorganizaciju stanica za formiranje funkcionalnog tkiva. Nosači su napravljeni od biokompatibilnih i/ili biorazgradljivih materijala. U zadnje vrijeme koriste se biorazgradljivi vlaknasti polimeri kao matrice za uzgoj i rast različitih tkivnih stanica. Posebice, nosači dobiveni elektroispredanjem poli(ε-kaprolaktona) (PCL-a) pokazuju dobar potencijal kao nosači tkiva za regeneraciju kože. U ovom radu postupkom elektroispredanja pripremljena su nosač od čistog PCL-a te nosači PCL-a u kombinaciji s fibroinom svile (FS) (18% PCL+50% FS), antibiotikom Cefuroximom (18 % PCL+25 % CFU) i nosači 18% PCL+50% FS uz dodatak 0,5 mas% mikro i nano TiO2. Istražen je utjecaj FS, CFU i TiO2 na morfološku stukturu, viskoelastična svojstva i hidrofobnost elektroispredenih vlaknastih nosača elektronskim pretražnim mikroskopom (SEM) , dinamičko mehaničkom analizom (DMA) te određivanjem kontaktnog kuta.

Abstract (english)

Electrospinning is process of nanofiber production using electrostatic forces. Human tissue is prone to damage. Smaller wounds, scratches and sprains, and even milder fractures of the bones are easily healed. The problem are completely damaged organs that lose function as well as large-scale bone fractures. In addressing this problem, tissue engineering has a solution. Tissue engineering and manufacturing of functional tissues and organs are part of modern biomedical research and application. In tissue engineering cells are planted in biomaterials prior to transplantation. These materials then serve as temporary carriers that improve cell reorganization to form functional tissue. The carriers are made of biocompatible and or biodegradable materials. Recently, biodegradable fiber polymers are used as matrices for the cultivation and growth of different tissue cells. Specifically, scaffolds obtained by electrospinning of poly (ε-caprolactone) (PCL) exhibit good potential as skin regeneration tissue carriers. In this work by electrospinning procedure scaffolding materials were produced: polycaprolactone (PCL) scaffold (100% PCL), combined bi-polymer scaffold silk fibroin (SF) (18% PCL+50% FS), (PCL) scaffold with antibiotic Cefuroxim (18 % PCL+25 % CFU) and 18% PCL+50% SF with incorporation of 0,5 mas.% of micro and nano TiO2. Morphology, viscoelastic properties and hydrofobicity of the scaffolds were characterized to evaluate the effect of FS,CFU and TiO2 by means of scanning electron microscopy (SEM), dynamic mechanical analysis and contact angle.

• Keywords: elektroispredanje; tkivno inženjerstvo; polikaprolakton; fibroin svile; titanijev dioksid
• Keywords (english): electrospinning; tissue engineering; polycaprolactone; silk fibroin; titanium dioxide
• Language: croatian
• URN:NBN: urn:nbn:hr:149:959135
• Project: Number: IP-2016-06-6878; Title: Ciljana izrada prototipa vlaknastog nosača za uzgoj tkivnih stanica kombiniranim elektroispredanjem; Acronym: COMBOELECTROSPUN; Leader: Budimir Mijović; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Materials Science and Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-27 16:34:49