Razvoj i validacija kromatografske metode za određivanje diklofenaka u vodi

undergraduate thesis

• Title (english): Development and validation of chromatographic method for the determination of diclofenac in water
• Author: Petra Vukovinski
• Mentor: Dragana Mutavdžić Pavlović (mentor)
• Committee member: Dragana Mutavdžić Pavlović (predsjednik povjerenstva)
• Committee member: Sandra Babić (član povjerenstva)
• Committee member: Marijana Hranjec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Validacija, tj. vrednovanje svih bitnih postupaka analitičkog sustava, predstavlja prvi korak kojim se određuje i dokumentira prikladnost analitičkog sustava za određenu namjenu. Kako bi validirali analitički sustav potrebno je ispitati izvedbene značajke kao što su: linearnost, točnost, specifičnost/selektivnost, granicu detekcije i granicu kvantifikacije, robusnost, radno područje, preciznost i osjetljivost. Cilj ovoga rada bio je osmisliti i validirati kromatografsku metodu za određivanje farmaceutika diklofenaka u izvorskoj i MilliQ vodi te se upoznati s izvedbenim značajkama analitičkog sustava. U ovome je radu opisana priprema otopina diklofenaka u izvorskoj i MilliQ vodi te je analiza provedena na tekućinskom kromatografu visoke učinkovitosti uz detektor s nizom dioda (HPLC-DAD). Rezultati analize potvrdili su da je ispitana kromatografska metoda valjana za određivanje diklofenaka u vodi jer su gotovo sve izvedbene značajke zadovoljile kriterij.

Abstract (english)

Validation, i.e., evaluation of all relevant procedures of the analytical system, represents the first step to determining and documenting the suitability of the analytical system for a specific purpose. Performance parameters such as linearity, accuracy, specificity/selection, limit of detection and limit of quantitation, robustness, work area, precision, and sensitivity need to be examined to validate. This work aims to develop and validate a chromatographic method for the determination of diclofenac in spring water and MilliQ water and become familiar with the performance parameters of the analytical system. In this work, the preparation of diclofenac solutions in spring water and MilliQ water is described. The analysis was performed on a high-performance liquid chromatograph coupled to a diode array detector (DAD). The research results have confirmed that the tested method is acceptable for the determination of diclofenac in water because almost all parameters met the criterion.

• Keywords: validacija kromatografske metode; tekućinska kromatografija visoke učinkovitosti; diklofenak; izvedbene značajke
• Keywords (english): validation of chromatographic method; high performance liquid chromatography; diclofenac; performance parameters
• Language: croatian
• URN:NBN: urn:nbn:hr:149:263701
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-02-28
• Created on: 2023-03-15 09:51:08