Fino strukturno ugađanje heterocikličkih boja na bazi stirila za spektrofotometrijsko prepoznavanje različitih ds-DNA/RNA slijedova

master's thesis

• Title (english): Fine structural tuning of heterocyclic dyes with a styryl base for spectrophotometric recognition of different ds-DNA/RNA sequences
• Author: Ivona Pečurlić
• Mentor: Irena Škorić (mentor)
• Mentor: Ivo Piantanida (komentor)
• Committee member: Irena Škorić (predsjednik povjerenstva)
• Committee member: Željka Ujević Andrijić (član povjerenstva)
• Committee member: Ivo Piantanida (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-09-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

U sklopu ovog diplomskog rada ispitivane su interakcije s molekulama DNA i RNA za četiri stirilne boje, različitih heterocikličkih jezgri te položaja supstituenata. Sve boje imaju strukturu koja sadržava konjugirani sustav te uz pozitivan naboj, pokazuju odlični potencijal za vezanje na polinukleotide. Svi spojevi su karakterizirani uz metode UV/Vis spektrofotometrije i spektrofluorimetrije te preliminarnim ispitivanjima vezanja na DNA, uočena je značajna promjena fluorescencijskog odziva kao naznaka vezanja. UV/Vis te fluorimetrijskim titracijama spojeva s polinukleotidima, ustanovljeno je kako svi spojevi pokazuju afinitet prema vezanju na RNA te ponešto viši afiniteti vidljivi su prema DNA, što je izraženo logaritmom konstante stabilnosti određenih prema Scatchardovoj jednadžbi. Opisujući način vezanja, sve boje pokazuju nespecifično vezanje u utore. Spojevi pokazuju slab stabilizacijski utjecaj na polinukleotide, u većoj mjeri na RNA nego na DNA molekulu.

Abstract (english)

Within this Master's thesis, interactions with DNA and RNA molecules were investigated for four styryl dyes, with different heterocyclic cores and substituent positions. All dyes have a structure that contains a conjugated system and a positive charge, which shows excellent potential for binding to polynucleotides. All compounds were characterized with the UV/Vis spectrophotometry and spectrofluometry methods, and with preliminary DNA binding tests, a significant change in fluorescence response was observed as an indication of binding. By UV/Vis and fluorimetric titrations of compounds with polynucleotides, it was established that all compounds show affinity towards binding to RNA, and slightly higher affinities are visible towards DNA, which are expressed by the logarithm of the stability constant determined according to Scatchard's equation. Describing the mode of binding, all dyes show non-specific binding to the grooves. The compounds show a weak stabilizing effect on polynucleotides, to a greater extent on RNA than on DNA molecules.

• Keywords: stirilne boje; DNA; RNA; UV/Vis spektrofotometrija; spektrofluorimetrija; cirkularni dikroizam
• Keywords (english): styryl dyes; DNA; RNA; UV/Vis spectrophotometry; spectrofluorimetry; circular dichroism
• Language: croatian
• URN:NBN: urn:nbn:hr:149:809039
• Project: Number: IP-2018-01-5475; Title: Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteina; Acronym: BioMultiChromoProbes; Leader: Ivo Piantanida; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-03-19 17:19:11