Optimizacija procesa ekstrakcije polifenola

master's thesis

• Title (english): Optimization of polyphenol extraction
• Author: Ana Juričić
• Mentor: Jasna Prlić Kardum (mentor)
• Committee member: Jasna Prlić Kardum (predsjednik povjerenstva)
• Committee member: Aleksandra Sander (član povjerenstva)
• Committee member: Mirela Leskovac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Polifenoli su biološki aktivni spojevi prisutni u velikom broju biljaka te im je svrha zaštititi biljku od negativnih vanjskih utjecaja. Zbog anti-age svojstava polifenola posljednjih nekoliko godina istražuje se njihova primjena u kozmetičkoj industriji. Zato je cilj ovog rada bila priprema DES-a i odabir odgovarajuće metode za ekstrakciju polifenolnih spojeva iz lista trputca. Pripremljeno je 19 otapala od kojih je njih 12 pokazalo dobra fizikalno-kemijska svojstva za daljnju primjenu u procesu ekstrakcije. Za odabrana otapala određena su fizikalno-kemijska svojstva (gustoća, viskoznost, pH i polarnost), snimljeni FTIR spektri te je nakon provedene ekstrakcije određena koncentracija ukupnih fenola. Uz to, praćen je utjecaj dodatka vode na učinkovitost ekstrakcije i fizikalna svojstva DES-ova. Najbolju učinkovitost ekstrakcije pokazao je DES La:Fru:Gly (2:1:2) s 30 i 50 mas% vode te je za La:Fru:Gly s
50 mas% vode provedena optimizacija procesa. Određena je najbolja metoda ekstrakcije te vrijeme potrebno za postizanje ravnotežne koncentracije polifenola. Nadalje je za odabrano otapalo određena ravnotežna koncentracija polifenola na granici faza te je određen difuzijski koeficijent i koeficijent prijenosa tvari.

Abstract (english)

Polyphenols are biologically active compounds present in a large number of plants and their purpose is to protect the plant from negative external influences. Due to the anti-age properties of polyphenols, their application in the cosmetics industry has been investigated in recent years. Therefore, this work aimed to prepare DES and select an appropriate method for the extraction of polyphenolic compounds from plantain leaves. 19 solvents were prepared, 12 of which showed good physicochemical properties for further use in the extraction process. Physicochemical properties (density, viscosity, pH, and polarity) were determined for the selected solvents, FTIR spectra were recorded and the concentration of total phenols was determined after the extraction. In addition, the influence of water addition on the extraction efficiency and physicochemical properties of DESs was monitored. The best extraction efficiency was shown by DES La: Fru: Gly (2: 1: 2) with 30 and 50 wt% water and for La: Fru: Gly - 50 wt% process optimization was performed. The best extraction method and the time for achieving equilibrium polyphenol concentration were determined. Furthermore, the equilibrium concentration of polyphenols at the phase boundary was determined for the selected solvent, and the diffusion coefficient and the transfer coefficient of the substance were determined.

• Keywords: ekstrakcija kapljevina-krutina; list trputca; niskotemperaturna eutektička otapala; polifenolni spojevi
• Keywords (english): liquid-solid extraction; plantain leaf; low-temperature eutectic solvents; polyphenolic compound
• Language: croatian
• URN:NBN: urn:nbn:hr:149:069805
• Project: Number: IP-2018-01-6504; Title: Ekstrakcija bioaktivnih biljnih sastavnica pomoću zelenih otapala – korak prema zelenim kozmeceuticima; Title: Bioactive plant principles extraction using green solvents - a step towards green cosmeceuticals; Acronym: CosmoGreen; Leader: Marijana Zovko Končić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-04-14 12:21:43