Regeneracija koenzima NAD(P)+ u reakcijama oksidacije katalizirane oksidoreduktazama

master's thesis

• Title (english): Regeneration of NAD(P)+ in the oxidation reactions catalyzed by oxidoreductases
• Author: Antonia Škarica
• Mentor: Ana Vrsalović Presečki (mentor)
• Committee member: Ana Vrsalović Presečki (predsjednik povjerenstva)
• Committee member: Vanja Kosar (član povjerenstva)
• Committee member: Anita Šalić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Industrijski procesi temeljeni na biokatalizi korištenjem enzima ili cijelih stanica smatraju se ekološki prihvatljivom tehnologijom. Oksidoreduktaze su jedna od najvećih skupina enzima (25 % svih enzima) i imaju veliku industrijsku i znanstvenu važnost. Glavna prepreka njihovoj široj primjeni je činjenica da ovi enzimi zahtijevaju kofaktore koji se troše tijekom reakcije, pri čemu je potrebno pronaći odgovarajući način za njihovu regeneraciju. Koenzimi NAD+ i NADP+ služe kao oksidansi koji se reduciraju u NAD(P)H dok se supstrat oksidira u ciljni produkt. U ovom radu provedeno je istraživanje potencijalne upotrebe enzima NAD(P)H oksidaze (NOX) za regeneraciju koenzima u reakcijama oksidacije. Budući da je upotrijebljeni enzim aktivan u reakcijama oksidacije koenzima NADH i NADPH, provedene su reakcije oksidacije supstrata etanola i 2-propanola s dva različita enzima i određen je ukupni broj obrtaja koenzima u svakoj reakciji. Izmjerena je kinetika svih enzima te je uspostavljen i validiran procesni model u kotlastom bioreaktoru. Rezultati pokazuju da se NOX može uspješno primjeniti za regeneraciju koenzima, pri čemu je enzim učinkovitiji prilikom regeneracije NADP+.

Abstract (english)

Industrial processes based on biocatalysis using enzymes or whole cells are considered environmentally friendly technology. Oxidoreductases are one of the largest classes of enzymes (25 % of all enzymes) and have far-reaching industrial and scientific importance. The main obstacle to their wider application is the fact that these enzymes require cofactors that are consumed during the reaction and it is necessary to find a suitable way for their regeneration. The coenzymes NAD+ and NADP+ serve as oxidants that are reduced to NAD(P)H while the substrate is oxidized to the target product. This study investigated the potential use of regenerating coenzymes in oxidation reactions using the enzyme NAD(P)H oxidase (NOX). Ethanol and 2-propanol oxidation processes were carried out with two enzymes, and the total turnover number of coenzymes in each reaction was calculated.
The kinetics of all enzymes were measured and a process model was established and validated in a batch bioreactor. The findings indicate that the application of NOX for the regeneration of coenzymes is feasible, with NADP+ showing significantly better results.

• Keywords: biokataliza; regeneracija koenzima; NAD(P)H; oksidoreduktaza; oksidacija; kinetika; matematičko modeliranje
• Keywords (english): biocatalysis; coenzyme regeneration; NAD(P)H; oxidoreductase; oxidation; kinetics; mathematical modelling
• Language: croatian
• URN:NBN: urn:nbn:hr:149:503738
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-10-01
• Created on: 2023-09-14 13:54:07