Kinetička karakterizacija aldolaze imobilizirane na magnetske nanočestice

master's thesis

• Title (english): Kinetic characterisation of aldolase immobilized on magnetic nanoparticles
• Author: Ivana Petrić
• Mentor: Ana Vrsalović Presečki (mentor)
• Committee member: Ana Vrsalović Presečki (predsjednik povjerenstva)
• Committee member: Martina Sudar (član povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Cilj ovog rada bio je odrediti kinetiku kovalentno imobiliziranog enzima DERA u reakciji dvostruke aldolne adicije acetaldehida i kloroacetaldehida. Produkt navedene reakcije je laktol – ključni prekursor u sintezi bočnog lanca statina, lijeka za snižavanje koncentracije kolesterola u krvi. Kao nosioc za imobilizaciju, korištene su magnetske nanočestice, sintetizirane hidrotermalnom metodom, funkcionalizirane 3-aminopropiltrietoksisilanom te aktivirane 10 %-tnim anhidridom jantarne kiseline. Kinetika je za oba koraka reakcije opisana Michaelis-Menteničinom jednadžbom, a kinetički su parametri procijenjeni metodom nelinearne regresije programa MicroMath Scientist. Uz to, razvijen je matematički model procesa i validiran u kotlastom reaktoru.

Abstract (english)

The aim of this work was to determine the kinetics of the covalently immobilized DERA enzyme in the double aldol addition reaction of acetaldehyde and chloroacetaldehyde.
The product of the mentioned reaction is lactol – a key precursor in the synthesis of the side chain of statin, a drug used for lowering blood cholesterol level. Magnetic nanoparticles were used as a carrier and were synthesized by the hydrothermal method, functionalized with 3-aminopropyltriethoxysilane and activated with 10 % succinic anhydride. The kinetics for both reaction steps was described by the Michaelis-Menten equation, and the kinetic parameters were estimated using the nonlinear regression method of the MicroMath Scientist software. A mathematical model of the process was also developed and validated in a batch reactor.

• Keywords: 2-deoksiriboza-5-fosfat aldolaza; imobilizacija; magnetske nanočestice; statin; kinetika
• Keywords (english): 2-deoxyribose-5-phosphate aldolase; immobilization; magnetic nanoparticles; statin; kinetics
• Language: croatian
• URN:NBN: urn:nbn:hr:149:197164
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-10-01
• Created on: 2023-11-20 14:35:53