Utjecaj uvjeta priprave na svojstva nosača temeljenih na poli(mliječnoj kiselini)

undergraduate thesis

• Title (english): Influence of preparation conditions on the properties of poly(lactic acid) based scaffolds
• Author: Marta Jerneić
• Mentor: Marica Ivanković (mentor)
• Committee member: Marica Ivanković (predsjednik povjerenstva)
• Committee member: Anamarija Rogina (član povjerenstva)
• Committee member: Krešimir Košutić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Inženjerstvo koštanog tkiva je multidisciplinarno područje čiji je cilj razviti materijale koji će zamijeniti oštećeno tkivo te potaknuti proces zacjeljivanja kosti. Jedan od potencijalnih sustava za takvu primjenu je kompozit kitozan/hidroksiapatit (CHT/HAp). Međutim njegova loša mehanička svojstva predstavljaju glavni nedostatak za primjene u inženjerstva tkiva. Poli(mliječna kiselina) (PLA) zbog svojih dobrih mehaničkih svojstava pozitivno utječe na svojstva kompozita. Prethodna istraživanja su pokazala da prilikom razvoja sustava
PLA/CHTHAp dolazi do gubitka mase, a samim time i do značajnog gubitka mehaničkih svojstava kompozitnog materijala. Stoga, cilj ovog završnog rada je ispitati utjecaj pojedinog koraka sinteze nosača temeljenog na poli(mliječnoj kiselini). PLA uzorci izloženi su octenoj kiselini i natrijevoj lužini u više vremenskih intervala (1h, 6h, 12h, 24h i 48h), te je ispitan utjecaj liofilizacije u dva vremenska intervala (24h i 48h). Nakon svakog koraka, gravimetrijskom analizom je utvrđen gubitak mase s ciljem određivanja koraka sinteze koji najznačajnije utječe na gubitak mase, te posljedično i gubitak mehaničkih svojstava
PLA/CHT-HAp kompozita. Rezultati istraživanja su pokazali da otopina octene kiseline nema značajnog utjecaja na PLA, dok otopina natrijeve lužine ubrzava proces degradacije polimera. Uzorci podvrgnuti utjecaju lužine okarakterizirani su metodom elektronske pretražne mikroskopije (SEM) i infracrvenom spektroskopijom s Fourierovim transformacijama (FTIR).

Abstract (english)

Bone tissue engineering is a multidisciplinary field aimed at developing materials that will replace damaged tissue and stimulate bone healing process. One potential system for such applications is the chitosan/hydroxyapatite (CHT/HAp) composite. Poly(lactic acid) (PLA), due to its good mechanical properties, positively affects the composites characteristic. Previous research has shown that during the development of the PLA/CHT-HAp scaffold, a certain weight loss occured and consequently, a significant loss of mechanical properties in the composite material was observed. Therefore, the goal of this experimental work is to examine the impact of individual synthesis steps on the properties of the poly(lactic acid) based scaffold. PLA samples were exposed to acetic acid and sodium hydroxide in several time intervals (1h, 6h, 12h, 24h and 48h), and the effect of lyophilization was examined for two time intervals (24h and 48h). After each step, the weight loss was determined by gravimetric analysis to identify the synthesis step that significantly affects weight loss and consequently the loss of mechanical properties of the PLA/CHT-HAp composite. The research results showed that acetic acid solution had no significant effect on PLA, while the sodium hydroxide solution accelerated the polymer degradation process. Samples subjected to the alkaline solution were characterized by scanning electron microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR).

• Keywords: poli(mliječna kiselina); degradacija; tkivno inženjerstvo
• Keywords (english): poly(lactic acid); degradation; tissue engineering
• Language: croatian
• URN:NBN: urn:nbn:hr:149:734154
• Project: Number: KK.01.1.1.07.0014; Title: Biorazgradive 3D tiskane strukture za augmentaciju kosti; Title: Biodegradable 3D printed structures for bone augmentation; Acronym: BIDEAS; Leader: Hrvoje Ivanković; Jurisdiction: eu; Funder: Europska unija; Funding stream: European Regional Development Fund
• Study programme: Title: Environmental Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-11-17 10:46:24