Kromatografske metode za praćenje fotokatalitičke razgradnje hidroksiklorokina

master's thesis

• Title (english): Chromatographic methods for the monitoring of photocatalytic degradation of hydroxychloroquine
• Author: Martina Kocijan
• Mentor: Sandra Babić (mentor)
• Committee member: Sandra Babić (predsjednik povjerenstva)
• Committee member: Irena Škorić (član povjerenstva)
• Committee member: Davor Ljubas (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2018-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Analytic Chemistry

Abstract

U ovom radu razvijene su metode HPLC-DAD i HPLC-FLD za praćenje farmaceutika hidroksiklorokina u uzorcima vode. U okviru validacije razvijenih metoda ispitana je linearnost, osjetljivost i točnost, te su određene granice detekcije i kvantifikacije i radno područje metoda. Usporedbom izvedbenih značajki obje metode zaključeno je da je metoda HPLC-FLD prihvatljivija za analizu hidroksiklorokina jer omogućava određivanje nižih koncentracija ispitivanog farmaceutika. Obje razvijene metode primijenjene su za praćenje fotolitičke i fotokatalitičke razgradnje hidroksiklorokina. U drugom dijelu rada ispitana je fotolitička i fotokatalitička razgradnja hidroksiklorokina uz primjenu TiO2 kao fotokatalizatora u obliku tankog filma i suspenzije te različitih izvora zračenja: LED svjetiljka 365 nm i 405 nm, svjetiljka sa zračenjem sličnim Sunčevom i halogena svjetiljka. Rezultati fotolitičke i fotokatalitičke razgradnje hidroksiklorokina pokazali su da nije podložan fotolitičkoj razgradnji, a fotokatalitička razgradnja brža je uz fotokatlizator prisutan u obliku suspenzije. Kinetika fotokatalitičke razgradnje slijedi kinetiku prvog reda. Validiranom metodom HPLC-FLD uočeno je nastajanje četiri razgradna produkta tijekom fotokatalitičke razgradnje LED svjetiljkom 365 nm, dok se tijekom fotoktalitičke razgradnje uz osvjetljavanje LED svjetiljkom 405 nm javljaju tri razgradna produkta. Validiranom metodom HPLC-DAD uočeno je nastajanje dva razgradna produkta tijekom fotokatalitičke razgradnje uz osvjetljavanje LED svjetiljkom 405 nm, dok validiranom metodom uz osvjetljavanje LED svjetiljkom 365 nm nisu detektirani razgradni produkti.

Abstract (english)

In this paper, HPLC-DAD and HPLC-FLD methods have been developed to monitor the hydroxylchloroquine pharmaceuticals in water samples. Within the validation of the methods developed, linearity, sensitivity and accuracy were examined, and the limits of detection and quantification and the working range of the method were determined. By comparing the performance characteristics of both methods, it was concluded that the HPLC-FLD method is more acceptable for the analysis of hydroxychloroquine because it allows the determination of lower concentrations of the investigated drug. Both methods were used to monitor photolytic and photocatalytic degradation of hydroxychloroquines. In the second part, photolytic and photocatalytic degradation of hydroxychloroquine was investigated using TiO2 as photocatalysts in the form of thin film and suspensions and various sources of radiation: LED lamps at wavelengths of 365 and 405 nm, solar - like lamp and halogen lamp. The results of photolytic and photocatalytic degradation of hydroxychloroquines have shown that they are not subject to photolytic degradation, and photocatalytic degradation is faster with the photocatalyst present in the form of suspension. Photocatalytic degradation kinetics follows the kinetics of the first order. Using the validated HPLC-FLD method, four degradation products were detected during photocatalytic degradation by a 365 nm LED lamp, whilst during photocatalytic degradation with 405 nm LED lamp, there were three degradation products. Using the validated HPLC-DAD method, two degradation products were detected during photocatalytic degradation by a 405 nm LED lamp, whilst during photocatalytic degradation with 365 nm LED lamp did not detect any degradant products.

• Keywords: farmaceutici; hidroksiklorokin; tekućinska kromatografija; fotoliza; fotokataliza
• Keywords (english): pharmaceuticals; hydroxychloroquine; liquid chromatography; photolysis; photocatalysis
• Language: croatian
• URN:NBN: urn:nbn:hr:149:040705
• Study programme: Title: Materials Science and Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-27 13:28:00