Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala

master's thesis

• Title (english): Extraction of piperine from different types of peppers: green solvent screening
• Author: Andrea Opačak
• Mentor: Aleksandra Sander (mentor)
• Committee member: Aleksandra Sander (predsjednik povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Committee member: Jasna Prlić Kardum (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-01-27, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Kako bi se postigli ciljevi održivog razvoja, razvoj novih zelenih otapala trenutno je u žarištu istraživanja. Zbog lakog procesa pripreme, niske cijene, potencijalne biorazgradivosti i zanemarive toksičnosti niskotemperaturna eutektička otapala (engl. Deep Eutentic Solvents (DES)) prepoznata su kao dobra zamjena organskim otapalima. Zahvaljujući sve većem interesu i razvoju niskotemperaturnih eutektičkih otapala sve se više primjenjuju kao mediji za ekstrakciju, kromatografiju te u biomedicinske svrhe u proteklih nekoliko godina. Cilj ovog rada bio je utvrditi koje niskotemperaturno eutektičko otapalo je najpogodnije za ekstrakciju piperina iz bijelog i zelenog papra. Zrna papra usitnjena su i prosijana te su dobivene tri veličinske frakcije. Piperin je nepolarna molekula te su zbog toga za ekstrakciju su pripravljena četiri hidrofobna otapala, od kojih su dva na bazi timola te dva na bazi mentola. Modelom COSMO-RS predviđena je topljivost piperina u pripremljenim otapalima te je provedena ekstrakcija sa svim veličinskim frakcijama papra. Koncentracija piperina u dobivenim uzorcima utvrđena je UV/Vis spektroskopijom, bez prethodne obrade ekstrakata. Također, disk-difuzijskim testom ispitano je antimikrobno djelovanje ekstrakata, a test toksičnosti čistih otapala proveden je pomoću bioluminiscentne bakterije Vibrio fischeri.

Abstract (english)

In order to achieve the goals of sustainable development, the development of new green solvents is currently in the focus of research. Due to the easy preparation process, low price, potential biodegradability and negligible toxicity, Deep Eutentic Solvents (DES) are recognized as a good substitute for organic solvents. Thanks to the increasing interest and development of deep eutentic solvents, it is increasingly used as a medium for extraction, chromatography and for biomedical purposes in the past few years. The aim of this work was to determine which deep eutentic solvent is most suitable for the extraction of piperine from white and green pepper. The peppercorns were crushed and sieved, and three size fractions were obtained. Piperine is a non-polar molecule, which is why four hydrophobic solvents are prepared for extraction, two of them are thymol-based and two are menthol-based. The solubility of piperine in the prepared solvents was predicted by the COSMO-RS model, and extraction was performed with all size fractions of pepper. The concentration of piperine in the obtained samples was determined by UV/Vis spectroscopy, without prior processing of the extracts. Also, the antimicrobial activity of the extracts was tested with the disk diffusion test, and the toxicity test of pure solvents was performed using the bioluminescent bacterium Vibrio fischeri.

• Keywords: ekstrakcija; papar; piperin; niskotemperaturna eutektička otapala; antimikrobna aktivnost
• Keywords (english): extraction; pepper; piperine; deep eutentic solvents; antimicrobial activity
• Language: croatian
• URN:NBN: urn:nbn:hr:149:238036
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-02-15 11:43:16