Priprava i karakterizacija kompozitnog skeleta za potencijalnu primjenu u inženjerstvu koštanog tkiva

master's thesis

• Title (english): Preparation and characterization of composite scaffold for potential application in bone tissue engineering
• Author: Andrija Hanžek
• Mentor: Marica Ivanković (mentor)
• Committee member: Marica Ivanković (predsjednik povjerenstva)
• Committee member: Jelena Macan (član povjerenstva)
• Committee member: Hrvoje Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2015-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Chemical Engineering in Material Development

Abstract

Cilj rada bila je priprava bioaktivnog i biorazgradivog skeleta na temelju hidroksiapatita (HA), kitozana (CHT) i polilaktidnog (PLA) nosača za potencijalne primjene u inženjerstvu koštanog tkiva.
Napredak inženjerstva tkiva omogućen je primjenom biomaterijala trodimenzijskih struktura na kojima se, prije samog presađivanja, uzgajaju koštane stanice. Biorazgradivi polimerni materijali služe kao podloga (nosač) za širenje stanica te daju mehaničku čvrstoću novonastalom tkivu. U inženjerstvu koštanog tkiva, osim polimernih matrica koristi se i bioaktivna keramika kao što je hidroksiapatit. Hidroksiapatit je po svom kemijskom sastavu sličan anorganskom dijelu kosti (biološkom apatitu) i pozitivno utječe na umnažanje (proliferaciju) stanica.
Sinteza poroznog kompozitnog nosača PLA/CHT-HA s 30 % masenog udjela HA u kitozanskoj matrici provedena je metodom toplinski inducirane fazne separacije uz geliranje i ekstrakciju. Karakterizacija pripravljenih nosača utvrđena je instrumentalnim metodama: infracrvenom spektroskopijom s Fourierovim transformacijama, rendgenskom difrakcijskom analizom, pretražnom elektronskom mikroskopijom i diferencijalnom pretražnom kalorimetrijom. Kako bi se predvidjelo bubrenje materijala in vivo, kapacitet bubrenja nosača ispitan je u biološkom mediju. Test citotoksičnosti ekstrakta nosača proveden je na stanicama fibroblasta (MEH) nakon 3 i 7 dana uzgajanja. Karakterizacija kompozitnog nosača potvrdila je in situ nastajanje HA unutar kitozanske matrice, dobro prianjanje polimernih faza i visokoporoznu strukturu PLA/CHT-HA nosača. Analiza citotoksičnosti potvrdila je da pripravljeni nosači nemaju štetan utjecaj na stanice.

Abstract (english)

The aim of this work was preparation of bioactive and biocompatibile scaffold based on hydroxyapatite (HA), chitosan (CHT) and polylactide (PLA) scaffold with potential application in bone tissue engineering.
The progress of tissue engineering is possible by the application of 3D biomaterials structures seeded with bone cells prior to transplantation. Biodegradable polymeric materials are used as scaffolds for cell proliferation and as mechanical support for new tissue. Besides polymeric matrices, bioactive ceramics such as hydroxyapatite are used in bone tissue engineering. Hydroxyapatite possesses chemical similarity to the inorganic phase of the bone (biological apatite) and has positive effect on cell proliferation.
Synthesis of porous composite PLA/CHT-HA scaffold with 30 mass% of HA within chitosan matrix has been carried out by freeze-gelation method. Characterization of prepared scaffolds was carried out by instrumental methods: FTIR spectroscopy, X-ray diffraction analysis, SEM microscopy and differential scanning calorimetry. To predict the swelling behaviour of materials in vivo, swelling capacity was assessed in biological medium. Cytotoxicity assay on fibroblast cells was carried out by the scaffolds supernatant after 3 and 7 days of culture. Characterization of composite scaffold has confirmed in situ HA formation within chitosan matrix, good adhesion between polymeric phases and highly porous structure of PLA/CHT-HA scaffold. Cytotoxicity assay of prepared scaffolds has confirmed no harmful effect on cells.

• Keywords: hidroksiapatit (HA); kitozan (CHT); polilaktid (PLA); skelet (nosač); citotoksičnost
• Keywords (english): hydroxyapatite (HA); chitosan (CHT); polylactide (PLA); scaffold; cytotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:149:598565
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-12-07 13:46:04