Optimiranje hidrolize proteina u ljuskama kozica

master's thesis

• Title (english): Optimisation of protein hydrolysis in prawns shell
• Author: Sara Čačko
• Mentor: Ana Vrsalović Presečki (mentor)
• Committee member: Ana Vrsalović Presečki (predsjednik povjerenstva)
• Committee member: Vanja Kosar (član povjerenstva)
• Committee member: Zlata Hrnjak-Murgić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-09-17, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Proteaze su jedne od najvažnijih skupina industrijskih enzima koje kataliziraju razgradnju proteina. Zbog visokog prinosa, malog utroška energije, niskih troškova proizvodnje i jednostavne genetičke manipulacije proteaze se koriste pri deproteinizaciji morskih ostataka u svrhu dobivanja hitina. U okviru ovoga rada provedena je optimizacija hidrolize proteina iz ljusaka kozica pri optimalnim uvjetima rada ispitanih proteaza (T = 40°C i pH = 8). Hidroliza proteina katalizirana je komercijalnim proteazama Flavourzyme, Novozyme, Neutraza i Novo-pro D pri koncentracijama 0.5 U / 10 mg i 0.1 U / 10 mg otpada. Za navedene proteaze ispitana je operacijska aktivnost te se zaključilo da troparametarski model najbolje opisuje inaktivaciju proteaza. Pri hidrolizi proteina iz ostataka kozica sve proteaze pri obje koncentracije su se pokazale uspješnima, a najučinkovitije i najstabilnije su bile Novo-pro D i Novozyme zbog čega su se s njima proveli daljnji eksperimenti s ciljem utvrđivanja koliko se puta ista proteaza može upotrijebiti za hidrolizu proteina. Iz eksperimenata u ponavljajućem rektoru s proteazama Novo-pro D i Novozyme, pri koncentracijama 0.5 U / 10 mg i 0.1 U / 10 mg, pokazalo se da se proteaze mogu upotrijebiti u više ciklusa (do 5 ciklusa pri koncentraciji 0.5 U / 10 mg, a pri koncentraciji i 0.1 U / 10 mg do 3-4 ciklusa).

Abstract (english)

Proteases are one of the most important groups of industrial enzymes that catalyze the the protein degradation. Due to their high yield, low energy consumption, low production costs and simple genetic manipulation, proteases are used in the deproteinization of marine residues for the purpose of obtaining chitin. In this work, the optimization of protein hydrolysis from prawn shells was performed under optimal operating conditions of the tested proteases (T = 40°C and pH = 8). Protein hydrolysis was catalyzed by the commercial proteases Flavourzyme, Novozyme, Neutrase, and Novo-pro D at concentrations of 0.5 U / 10 mg and 0.1 U / 10 mg of waste. For these proteases, the operation stability was examined, and it was concluded that the three-parameter model best describes the inactivation of proteases. In the hydrolysis of proteins from prawn residues, all proteases at both concentrations proved successful, and Novo-pro D and Novozyme were the most efficient and stable and was used in repetitive to determine how many times the same protease can be used for protein hydrolysis. In repetitve experiments with proteases Novo-pro D and Novozyme, used at concentrations of 0.5 U / 10 mg and 0.1 U / 10 mg, it was shown that proteases can be used in several cycles (up to 5 cycles at a concentration of 0.5 U / 10 mg, and at a concentration of 0.1 U / 10 mg for up to 3-4 cycles).

• Keywords: hidroliza proteina; proteaze; ostaci kozica; hitin
• Keywords (english): hydrolysis of protein; protease; prawn waste; chitin
• Language: croatian
• URN:NBN: urn:nbn:hr:149:017476
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-10-01
• Created on: 2023-04-14 10:07:34