Enzimatska hidroliza proteina u otpadu ljuski rakova

master's thesis

• Title (english): Enzymatic hydrolysis of protein in shrimp waste
• Author: Petra Bingula
• Mentor: Ana Vrsalović Presečki (mentor)
• Committee member: Ana Vrsalović Presečki (predsjednik povjerenstva)
• Committee member: Ana Lončarić Božić (član povjerenstva)
• Committee member: Marin Kovačić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Enzimi su proteini čija je osnovna zadaća ubrzavanje biokemijskih reakcija od kojih je svaka katalizirana jedinstvenim enzimom. Proteaze su jedna od najvažnijih skupina enzima, a njihova primjena svakodnevno raste u industriji. Razlog tome je ekonomska isplativost u vidu niskih troškova proizvodnje i male potrošnje energije, visokih prinosa i jednostavnosti genetičkih modifikacija. U ovom radu je ispitana učinkovitost enzima u hidrolizi proteina iz otpada kozica primjenom šest komercijalnih proteaza: Alkalaza, Neutraza, Esperaza, Flavourzyme, Novozyme i Novo-ProD. Svi navedeni enzimi su pokazali uspješnost u razgradnji proteina. Utvrđeno je kako su provedbom hidrolize i pri manjim koncentracijama enzima konverzije gotovo jednako visoke u većini slučajeva, osim kod Neutraze gdje je konverzija nešto niža. Kod većine enzima su visoke konverzije postignute već u prvom satu, što kod Neutraze nije slučaj budući da je konverzija postupno rasla. Po završetku hidrolize proteina Alkalaza, Flavourzyme i Novo-ProD pokazali su najveće stabilnosti pri koncentraciji enzima od 0,5 U / 10 mg, stoga su odabrani za provedbu hidrolize proteina u ponovljivom reaktoru sa 6 ciklusa. Pritom su Alkalaza i Novo-ProD pokazali zadovoljavajuće rezultate u smislu visokih konverzija te aktivnosti nakon zadnjeg ciklusa.

Abstract (english)

Enzymes are proteins whose primary task is to catalyse biochemical reactions, where each is catalyzed by a unique enzyme. Proteases are one of the most important groups of enzymes, and their application in industry is growing daily. The reason for this is economic profitability due to low production costs and low energy consumption, high yields and simplicity of genetic modifications. In this paper, the efficiency of enzymes in the hydrolysis of proteins from shrimp waste was examined using six commercial proteases: Alcalase, Neutrase, Esperase, Flavourzyme, Novozyme and Novo-ProD. All enzymes were successful in protein degradation. It was found that by performing hydrolysis even at lower enzyme concentrations, the conversions are almost equally high in most cases, except by Neutrase where the conversion is a bit lower. For most enzymes, high conversions were achieved in the first hour of experiment, which was not the case with Neutrase as the conversion gradually increased. Upon completion of protein hydrolysis, Alcalase, Flavourzyme, and Novo-ProD showed the highest stability at an enzyme concentration of 0.5 U / 10 mg, and were therefore selected to catalyse protein hydrolysis in a repetitive reactor with six cycles. Alcalase and Novo-ProD showed satisfactory results in terms of high conversions and activity after the last cycle.

• Keywords: enzimi; proteaze; otpad kozica; hidroliza proteina; hitin; konverzija
• Keywords (english): enzymes; proteases; shrimp waste; protein hydrolysis; chitin; conversion
• Language: croatian
• URN:NBN: urn:nbn:hr:149:248258
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-10-01
• Created on: 2023-04-14 11:59:51