Antioksidativna aktivnost eteričnih ulja

master's thesis

• Title (english): Antioxidant activity of essential oils
• Author: Antonela Radetić
• Mentor: Aleksandra Sander (mentor)
• Committee member: Aleksandra Sander (predsjednik povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2024-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Eterična ulja imaju dugu povijest primjene, datirajući unatrag do samih početaka civilizacije. Različita istraživanja i interes znanstvenika doveli su do razvoja brojnih metoda ekstrakcije eteričnih ulja, koja su danas integralni dio svakodnevnog života. Zbog svojih ljekovitih svojstava eterična ulja nalaze široku primjenu u farmaceutskoj i prehrambenoj industriji, te su ključne komponente u industriji parfema i kozmetike. U ovom radu ispitan je kemijski sastav i antioksidacijska aktivnost uzoraka eteričnih ulja ekstrahiranih hidrodestilacijom po Clevengeru. Eterična ulja ekstrahirana su iz kore limuna (Citrus limon), lovorovog lista (Laurus nobilis), klinčića (Syzygium aromaticum), muškatnog oraščića (Myristica fragrans Houtt – officinalis L.), zvjezdastog anisa (Pimpinella anisum) i origana (Origanum vulgare).
Na temelju provedene ekstrakcije dani su podaci o prinosu svake vrste eteričnog ulja. Karakterizacija uzoraka provedena je tankoslojnom kromatografijom, sustavom plinske kromatografije, infracrvenom spektroskopijom s Fourierovom transformacijom. Za svaki uzorak ekstrahiranog eteričnog ulja identificirana je najzastupljenija komponenta. Ispitanim uzorcima određen je udio ukupnih prisutnih fenola metodom s Folin – Ciocalteu reagensom, a zatim je DPPH i ABTS metodama određena antioksidacijska aktivnost.

Abstract (english)

Essential oils have a long history of use, dating back to the beginnings of civilization. Various research efforts and scientific interest have led to the development of numerous extraction methods for essential oils, which are now an integral part of everyday life. Due to their medicinal properties, essential oils are widely used in the pharmaceutical and food industries and are also important ingredients in the perfume and cosmetics industries. In this study, the chemical composition and antioxidant activity of essential oil samples extracted by Clevenger hydrodistillation are investigated. Essential oils were extracted from lemon peel (Citrus limon), laurel (Laurus nobilis), clove (Syzygium aromaticum), nutmeg (Myristica fragrans Houtt – officinalis L.), star anise (Pimpinella anisum) and oregano (Origanum vulgare).
The data on the yield of the individual types of essential oils originate from the extraction carried out. The samples were characterised using thin-layer chromatography, gas chromatography and Fourier transform infrared spectroscopy. For each extracted essential oil sample, the most abundant component was identified. The total phenolic content of the analysed samples was determined using the Folin-Ciocalteu reagent method, followed by the determination of the antioxidant activity using the DPPH and ABTS methods.

• Keywords: eterično ulje; hidrodestilacija po Clevengeru; antioksidacijska aktivnost; Citrus limon; Laurus nobilis; Syzygium aromaticum; Myristica fragrans Houtt – officinalis L.; Pimpinella anisum; Origanum vulgare
• Keywords (english): essential oil; Clevenger hydrodistillation; antioxidant activity; Citrus limon; Laurus nobilis; Syzygium aromaticum; Myristica fragrans Houtt – officinalis L.; Pimpinella anisum; Origanum vulgare
• Language: croatian
• URN:NBN: urn:nbn:hr:149:109243
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-15 11:27:37