Analiza kemoterapeutika u okolišu kromatografskim metodama

master's thesis

• Title (english): Analysis of chemotherapeutic agents in the environment by chromatographic methods
• Author: Jelena Perčić
• Mentor: Danijela Ašperger (mentor)
• Committee member: Danijela Ašperger (predsjednik povjerenstva)
• Committee member: Tatjana Gazivoda Kraljević (član povjerenstva)
• Committee member: Dragana Mutavdžić Pavlović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2016-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Analytic Chemistry

Abstract

Kemoterapeutici su kemijske tvari koje se koriste u liječenju bolesti. Mnogi kemoterapeutici prepoznati su kao potencijalna zagađivala okoliša diljem svijeta. Kemoterapeutici se mogu pronaći u okolišu zbog svoje velike proizvodnje i uporabe. Posljednjih godina njihova potrošnja se povećala zbog čega postaju sve veći problem jer mogu završiti u podzemnim i površinskim vodama, tlu i sedimentu. Na taj način mogu utjecati na zdravlje ljudi i na organizme u vodama. Najčešće se određuju kromatografskim metodama koje omogućuju istovremeno određivanje velikog broja kemoterapeutika, no zahtijevaju sofisticiranu pripravu uzorka. Kemoterapeutici ispitivani u ovom radu su iz grupe antihelmintika ili antiparazitika (albendazol, febantel, prazikvantel), antibiotika (nitrofurantoin, cefdinir), kemoterapeutika (hidroksiklorokin) i antiemetika (metoklopramid). Cilj ovog rada je optimizacija uvjeta za ekstrakciju kemoterapeutika iz sedimenta metodom raspršenjem matice uzorka kroz čvrstu fazu (MSPD), odnosno pronaći optimalno ekstrakcijsko otapalo. Ekstrakti su analizirani tekućinskom kromatografijom visoke djelotvornosti s detektorom s nizom dioda (HPLC-DAD). Najbolji rezultati za ekstrakciju kemoterapeutika iz sedimenta dobiveni su čistim otapalom etil-acetatom. Metoda je validirana određivanjem ponovljivosti, linearnosti, granice detekcije i granice kvantifikacije.

Abstract (english)

Many chemotherapeutic agents have been detected as aquatic pollutants all over the world. Chemotherapeutic agents are expected to be found in environment based on their big production and use. Last few years is increased their consumption. Because of that, they are becoming an increasing problem because they can end up in surface water, ground water, soil and sediment. Thus they can affect on human health and aquatic organisms. Chemotherapeutic agents are usually determined by chromatographic methods that allow the simultaneous determination of a large number of agents but demands a sophisticated sample preparation. Chemotherapeutic agents investigated in this study are from the group anthelmintics (albendazole, febantel, praziquantel), antibiotics (nitrofurantoin, cefdinir), chemotherapeutic agents (hydroxychloroquine) and antiemetics (metoclopramide). The aim of this work is optimization conditions for extraction of chemotherapeutic agents from sediment by matrix solid-phase dispersion (MSPD). The aim of optimization of method was find the optimal eluation solvent. The extract were analyzed by high-performance liquid chromatography with diode array detector (HPLC-DAD). The best results for extraction of chemotherapeutic agents from sediments are obtained with ethyl acetate. The MSPD-HPLC-DAD method was validated by determination of repeatibility, linearity, limit of detection and limit of determination.

• Keywords: farmaceutici; sediment; ekstrakcija raspršenjem uzorka kroz čvrstu fazu (MSPD); tekućinska kromatografija visoke djelotvornosti s detektorom s nizom dioda (HPLC-DAD)
• Keywords (english): pharmaceuticals; sediment; matrix solid-phase dispersion (MSPD); high-performance liquid chromatography with diode array detector (HPLC-DAD)
• Language: croatian
• URN:NBN: urn:nbn:hr:149:737531
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-04 12:03:03