Enzimatska sinteza 6-cijano-4-ketoheksan kiseline

master's thesis

• Title (english): Enzymatic synthesis of 6-cyano-4-ketohexanoic acidi
• Author: Anita Perešin
• Mentor: Zvjezdana Findrik Blažević (mentor)
• Committee member: Zvjezdana Findrik Blažević (predsjednik povjerenstva)
• Committee member: Martina Sudar (član povjerenstva)
• Committee member: Marija Vuković Domanovac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2017-07-03, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Reaction Engineering

Abstract

U ovom radu provedena je enzimska reakcija katalizirana enzimom sintaza 2-sukcinil-5-enolpiruvil-6-hidroksi-3-cikloheksen-1-karboksilne kiseline (MenD) s α-ketoglutaratom kao donorskim supstratom i akrilonitrilom kao akceptorskim supstratom, uz tiamin difosfat kao koenzim i magnezijev ion kao kofaktor. Reakcija je provedena u kotlastom reaktoru tijekom koje je praćena i aktivnost enzima. Osim toga proveden je i ponovljivi šaržni eksperiment. U ovom eksperimentu mjerena je koncentracija supstrata i produkta, te aktivnost enzima tijekom 72 sata. Nakon 24 i 48 sati u reaktor su dodani supstrati: α-ketoglutarat i akrilonitril kako bi se postigli isti početni uvjeti kao na početku reakcije. Također su provedeni i eksperimenti u kojima je ispitan utjecaj koncentracije akrilonitrila tijekom inkubacije na deaktivaciju enzima. Rezultati pokazuju da se tijekom provedbe reakcije smanjuje aktivnost enzima. U eksperimentima u kojima je praćen utjecaj koncentracije akrilonitrila na deaktivaciju enzima, rezultati pokazuju da se povećanjem koncentracije akrilonitrila smanjuje aktivnost enzima.

Abstract (english)

In this work, an enzymatic reaction catalyzed by 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic acid synthase (MenD) with α-ketoglutarate as donor substrate and acrylonitrile as acceptor substrate with thiamine diphosphate as coenzyme and magnesium ion as a cofactor was carried out. The reaction was carried out in a batch reactor during which the enzyme activity was monitored. In addition, a repetitive batch experiment was carried out. In this experiment, substrate and product concentration and enzyme activity were measured over 72 hours. After 24 and 48 hours fresh substrates α-ketoglutarate and acrylonitrile were added to achieve the same initial conditions as at the beginning of the reaction but without the addition of the enzyme. Also, the influence of acrylonitrile concentration during incubation on enzyme deactivation was investigated. The results show that during the reaction enzyme activity decreases. The influence of acrylonitrile concentration on enzyme deactivation show that increasing the concentration of acrylonitrile reduces enzyme activity.

• Keywords: MenD; Stetter reakcije; akrilonitril; α-ketoglutarat; kotlasti reaktor
• Keywords (english): MenD; Stetter reaction; acrylonitrile; α-ketoglutarate; batch reactor
• Language: croatian
• URN:NBN: urn:nbn:hr:149:089772
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-09-27 13:17:37