Karakterizacija 3D elektroispredenih materijala s različitom geometrijom

master's thesis

• Title (english): Characterization of 3D electrospun materials with different geometry
• Author: Nikolina Mrkonjić
• Mentor: Emi Govorčin Bajsić (mentor)
• Committee member: Emi Govorčin Bajsić (predsjednik povjerenstva)
• Committee member: Mirela Leskovac (član povjerenstva)
• Committee member: Veljko Filipan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2017-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Chemical Engineering in Material Development

Abstract

Elektroispredanje je tehnika koja koristi elektrostatske sile za dobivanje vlakana promjera raspona od 2 nm do nekoliko mikrometara iz polimernih otopina ili talina. Posljednjih desetljeća sve je veće istraživanje ove tehnike zbog primjene nanovlaknastih struktura kao nosača u tkivnom inženjerstvu. Strategija tkivnog inženjerstva temelji se na popravku i regeneraciji oštećenih tkiva na način da se najprije biopsijom stanice uzimaju iz pacijenta, potom izoliraju i zasijavaju na 3D nosač. Kao nosači se koriste različiti prirodni i umjetni polimeri. U ovom radu korišten je polikaprolakton (PCL) za elektroispredanje nosača različite geometrije. Elektroispredanjem PCL na rotirajućem cilindru nastao je nosač glatke površine koji je korišten kao referentni uzorak. Ostalih šest nosača elektroispredeno je na 3D printanim kolektorima različitih geometrija čime su dobivena tri nosača s paralelnim kanalićima i tri s kvadratnim udubinama. Ova specifična topografija elektroispredenih vlaknastih nosača potrebna je kako bi na njima zasijane fibroblastne stanice rasle i migrirale prema obliku površine nosača stvarajući tkivo određene 3D mikrogeometrije. Morfologija vlakana određena je SEM analizom, raspodjela veličina pora BET metodom, a interakcije na površini metodom mjerenja kontaktnog kuta s vodom. SEM analiza pokazala je veliku poroznost elektroispredenih nosača. BET metodom utvrđeno je da su srednji promjeri pora vrlo mali, ali je specifična površina izrazito velika. Rezultati mjerenja kontaktnog kuta pokazali su veliku hidrofobnost PCL nosača zbog čega je važno PCL nosače prije primjene obraditi kako bi im se povećala hidrofilnost i poboljšale interakcije stanica i nosača.

Abstract (english)

Electrospinning is a technique that uses electrostatic forces to produce fibers with diameters ranging from 2 nm to several micrometers from polymeric solutions or melts. Over the last decades, there is a growing research of technique because of the application of nanofibrous structures as scaffolds for tissue engineering. The tissue engineering strategy is based on the repair and regeneration of damaged tissues in way that we first take the cells from patient by biopsy, then we isolate and sow on the 3D scaffold. Various natural and synthetic polymers are used as scaffolds. In this study, polycaprolactone (PCL) was used for electrospinning of scaffolds with different geometry. By electrospinning process of PCL a scaffold with smooth surface was formed on rotating cylinder, which was used as a reference sample. The other six scaffolds are electrospun on 3D printed collectors with different geometries, resulting in three scaffolds with parallel ducts and three with quadratic wells. This specific topography of electrospun fibrous scaffolds is required to allow growth and migration of the sown fibroblast cells, forming a tissue of a certain 3D microgeometry. The fiber morphology was determined by SEM analysis, pore size distribution by BET method and interactions on surface were tested by the contact angle measurement method with water. The SEM analysis showed a high porosity of electrospun scaffolds. The BET method determined that the mean pore diameters are very small, but specific surface is very large.The result of the measurement of the contact angle showed a high hydrophobicity of the PCL scaffolds, which makes it important to treat the PCL scaffolds before its application, to increase hydrophilicity and improve the interaction of the cells and scaffolds.

• Keywords: elektroispredanje; nanovlakna; tkivno inženjerstvo; nosači; polikaprolakton; 3D printani kolektor
• Keywords (english): electrospinning; nanofibers; tissue engineering; scaffolds; polycaprolactone; 3D printed collector
• Language: croatian
• URN:NBN: urn:nbn:hr:149:736716
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-04-21 13:47:19